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PMID: 1826298 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Laminin receptors in the retina: sequence analysis of the chick integrin alpha 6 subunit. Evidence for transcriptional and posttranslational regulation.

The Journal of cell biology ·Vol. 113 ·No. 2 ·1991-04-00 ·Pages 405-16

de Curtis I, Quaranta V, Tamura RN, Reichardt LF

Abstract

The integrin alpha 6 beta 1 is a prominent laminin receptor used by many cell types. In the present work, we isolate clones and determine the primary sequence of the chick integrin alpha 6 subunit. We show that alpha 6 beta 1 is a prominent integrin expressed by cells in the developing chick retina. Between embryonic days 6 and 12, both retinal ganglion cells and other retinal neurons lose selected integrin functions, including the ability to attach and extend neurites on laminin. In retinal ganglion cells, we show that this is correlated with a dramatic decrease in alpha 6 mRNA and protein, suggesting that changes in gene expression account for the developmental regulation of the interactions of these neurons with laminin. In other retinal neurons the expression of alpha 6 mRNA and protein remains high while function is lost, suggesting that the function of the alpha 6 beta 1 heterodimer in these cells is regulated by posttranslational mechanisms.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chick Embryo Cloning, Molecular DNA Gene Expression Regulation Humans Integrins/genetics,metabolism Molecular Sequence Data Precipitin Tests Protein Biosynthesis Receptors, Immunologic/genetics,metabolism Receptors, Laminin Restriction Mapping Retina/growth & development,metabolism Retinal Ganglion Cells/metabolism Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Integrins Receptors, Immunologic Receptors, Laminin DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de Curtis I
Department of Physiology, University of California San Francisco 94143-0724.
Quaranta V
Tamura R N
Reichardt L F
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.113.2.405
Published
1991-04-00
Pages
405-16
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288948
Subset
IM
Grants
PHS HHS · 19090 · United States
Databases
GENBANK
X56559
External Links
PubMed source DOI: 10.1083/jcb.113.2.405 PMC Full Text View journal details
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