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PMID: 1693624 Published · ppublish English Journal Article

Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8.

The Journal of cell biology ·Vol. 110 ·No. 6 ·1990-06-00 ·Pages 2145-55

Sonnenberg A, Linders CJ, Modderman PW, Damsky CH, Aumailley M, Timpl R

Abstract

The involvement of integrins in mediating interaction of cells to well-characterized proteolytic fragments (P1, E3, and E8) of laminin was assessed by antibody blocking studies. Cell adhesion to fragment P1 was affected by mAbs against the integrin beta 1 and beta 3 subunits and furthermore could be prevented completely by a synthetic peptide containing the Arg-Gly-Asp sequence. Because the beta 3 antibody-sensitive cell lines expressed the vitronectin receptor (alpha v beta 3) at high levels, the involvement of this receptor in cell adhesion to P1 is strongly suggested. Integrin-mediated cell adhesion to E3 is of low affinity and was inhibited by antibodies against the integrin beta 1 subunit. In contrast, adhesion of some cell types to E3 was not or only partially sensitive to inhibition by anti-integrin subunit antibodies. Cell adhesion to E8 was blocked completed by integrin alpha 6 or beta 1 antibodies. The alpha 6-specific antibody did not inhibit cell adhesion to E3 or P1. Furthermore, the antibody only blocked adhesion to laminin of those cells that adhered exclusively to the E8 fragment. In addition, expression of alpha 6 beta 1 was closely correlated with the ability of cells to bind to the E8 fragment of laminin. These results indicate that the alpha 6 beta 1 integrin is a specific receptor for the E8 fragment of laminin. Many cell types expressed, instead of or in addition to alpha 6 beta 1 the recently described integrin alpha 6 beta 4. Although the ligand of alpha 6 beta 4 was not identified, it must be different from that of alpha 6 beta 1, because cells that express alpha 6 beta 4, but not alpha 6 beta 1, do not adhere to E8, and cell adhesion to E8 was specifically blocked by beta 1 specific antibodies. In conclusion, the data indicate that distinct integrin receptors belonging to the beta 1 or beta 3 subfamily are involved in adhesion of cells to the various laminin fragments. Adhesion to E3 may also be brought about by other receptor molecules, possibly proteoglycans, not belonging to the integrin family.

MeSH Terms
Animals Antibodies/immunology,physiology Breast/cytology,metabolism,ultrastructure Cell Adhesion/physiology Cell Line Female Humans Integrins/immunology,metabolism,physiology Laminin/analysis,immunology,metabolism Mammary Neoplasms, Experimental/metabolism,pathology,ultrastructure Melanoma/metabolism,pathology,ultrastructure Mice Ovarian Neoplasms/metabolism,pathology,ultrastructure Peptide Fragments/metabolism,physiology Receptors, Immunologic/metabolism,physiology Receptors, Laminin Receptors, Vitronectin
Chemicals
Antibodies Integrins Laminin Peptide Fragments Receptors, Immunologic Receptors, Laminin Receptors, Vitronectin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sonnenberg A
Department of Immunohaematology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Linders C J
Modderman P W
Damsky C H
Aumailley M
Timpl R
References (63)
63 references, click to expand
  1. The human laminin receptor is a member of the integrin family of cell adhesion receptors.
    Science. 1988 Sep 2;241(4870):1228-9 PMID: 2970671
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.
    J Cell Biol. 1989 Aug;109(2):877-89 PMID: 2547805
  4. The E8 subfragment of laminin promotes locomotion of myoblasts over extracellular matrix.
    J Cell Biol. 1989 Aug;109(2):799-809 PMID: 2503526
  5. Characterization of proteolytic fragments of the laminin-nidogen complex and their activity in ligand-binding assays.
    Eur J Biochem. 1988 Dec 1;178(1):71-80 PMID: 2462498
  6. Structural study of long arm fragments of laminin. Evidence for repetitive C-terminal sequences in the A-chain, not present in the B-chains.
    Eur J Biochem. 1988 Oct 15;177(1):35-45 PMID: 3181157
  7. Laminin-nidogen complex. Extraction with chelating agents and structural characterization.
    Eur J Biochem. 1987 Jul 1;166(1):11-9 PMID: 3109910
  8. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats.
    Proc Natl Acad Sci U S A. 1987 Feb;84(4):935-9 PMID: 3493487
  9. The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit.
    J Biol Chem. 1987 Mar 5;262(7):3300-9 PMID: 3546305
  10. Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures.
    J Biol Chem. 1988 Jun 5;263(16):7660-5 PMID: 2967289
  11. Evidence for coiled-coil alpha-helical regions in the long arm of laminin.
    EMBO J. 1985 Feb;4(2):309-16 PMID: 3848400
  12. Cell surface proteoglycan of mammary epithelial cells. Protease releases a heparan sulfate-rich ectodomain from a putative membrane-anchored domain.
    J Biol Chem. 1985 Apr 10;260(7):4103-9 PMID: 3156852
  13. Integrin heterodimer and receptor complexity in avian and mammalian cells.
    J Cell Biol. 1989 Jul;109(1):409-20 PMID: 2663881
  14. Adhesive protein receptors on hematopoietic cells.
    Immunol Today. 1988 Apr;9(4):109-13 PMID: 2855703
  15. The human fibroblast class II extracellular matrix receptor mediates platelet adhesion to collagen and is identical to the platelet glycoprotein Ia-IIa complex.
    J Biol Chem. 1988 Apr 5;263(10):4516-9 PMID: 2832397
  16. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    J Cell Biol. 1987 Oct;105(4):1873-84 PMID: 2822727
  17. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  18. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  19. Two distinct cell-binding domains in laminin can independently promote nonneuronal cell adhesion and spreading.
    J Cell Biol. 1987 Jul;105(1):589-98 PMID: 3038930
  20. Laminin receptor on platelets is the integrin VLA-6.
    Nature. 1988 Dec 1;336(6198):487-9 PMID: 2973567
  21. Identification of a neuronal laminin receptor: an Mr 200K/120K integrin heterodimer that binds laminin in a divalent cation-dependent manner.
    Neuron. 1988 Oct;1(8):713-25 PMID: 2978786
  22. A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor.
    Biochemistry. 1987 Nov 3;26(22):6896-900 PMID: 2962631
  23. The lymphocyte function-associated LFA-1, CD2, and LFA-3 molecules: cell adhesion receptors of the immune system.
    Annu Rev Immunol. 1987;5:223-52 PMID: 3109455
  24. The cellular interactions of laminin fragments. Cell adhesion correlates with two fragment-specific high affinity binding sites.
    J Biol Chem. 1987 Aug 25;262(24):11532-8 PMID: 3114248
  25. Role of laminin A chain in the development of epithelial cell polarity.
    Cell. 1988 Oct 21;55(2):331-41 PMID: 3048705
  26. Cytoadhesins, integrins, and platelets.
    Thromb Haemost. 1988 Feb 25;59(1):1-6 PMID: 3283991
  27. Mouse heart laminin. Purification of the native protein and structural comparison with Engelbreth-Holm-Swarm tumor laminin.
    J Biol Chem. 1989 Nov 5;264(31):18726-32 PMID: 2478549
  28. A novel vitronectin receptor integrin (alpha v beta x) is responsible for distinct adhesive properties of carcinoma cells.
    Cell. 1989 Apr 7;57(1):59-69 PMID: 2467745
  29. Tenascin mediates cell attachment through an RGD-dependent receptor.
    J Cell Biol. 1989 Mar;108(3):1149-55 PMID: 2466038
  30. Extracellular matrix receptors, ECMRII and ECMRI, for collagen and fibronectin correspond to VLA-2 and VLA-3 in the VLA family of heterodimers.
    J Cell Biochem. 1988 Aug;37(4):385-93 PMID: 2458366
  31. Identification and characterization of a novel antigen complex on mouse mammary tumor cells using a monoclonal antibody against platelet glycoprotein Ic.
    J Biol Chem. 1988 Oct 5;263(28):14030-8 PMID: 2459116
  32. Platelet glycoprotein IIb-IIIa-like proteins mediate endothelial cell attachment to adhesive proteins and the extracellular matrix.
    J Biol Chem. 1987 Jul 25;262(21):9935-8 PMID: 2440865
  33. Human endothelial cells synthesize and express an Arg-Gly-Asp-directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6471-5 PMID: 2442758
  34. Biosynthetic and functional properties of an Arg-Gly-Asp-directed receptor involved in human melanoma cell attachment to vitronectin, fibrinogen, and von Willebrand factor.
    J Biol Chem. 1987 Dec 25;262(36):17703-11 PMID: 2447074
  35. The human integrin VLA-2 is a collagen receptor on some cells and a collagen/laminin receptor on others.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9906-10 PMID: 2557634
  36. Identification of a cell-surface protein involved in PC12 cell-substratum adhesion and neurite outgrowth on laminin and collagen.
    J Neurosci. 1989 Sep;9(9):3287-96 PMID: 2552042
  37. Identification of integrin collagen receptors on human melanoma cells.
    J Biol Chem. 1989 Mar 15;264(8):4684-8 PMID: 2538453
  38. A novel integrin (alpha E beta 4) from human epithelial cells suggests a fourth family of integrin adhesion receptors.
    EMBO J. 1989 Mar;8(3):673-80 PMID: 2542022
  39. Endothelial cells use alpha 2 beta 1 integrin as a laminin receptor.
    J Cell Biol. 1989 Nov;109(5):2455-62 PMID: 2530239
  40. Human melanoma cells express a novel integrin receptor for laminin.
    J Biol Chem. 1989 Sep 15;264(26):15642-9 PMID: 2527855
  41. Peyer's patch-specific lymphocyte homing receptors consist of a VLA-4-like alpha chain associated with either of two integrin beta chains, one of which is novel.
    EMBO J. 1989 Jun;8(6):1735-41 PMID: 2670559
  42. Structure and biological activity of basement membrane proteins.
    Eur J Biochem. 1989 Apr 1;180(3):487-502 PMID: 2653817
  43. Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines.
    J Biol Chem. 1989 Apr 15;264(11):6529-35 PMID: 2649503
  44. The high-affinity binding of laminin to cells. Assignation of a major cell-binding site to the long arm of laminin and of a latent cell-binding site to its short arms.
    Eur J Biochem. 1989 Mar 1;180(1):9-14 PMID: 2651126
  45. Human vascular endothelial cells express a membrane protein complex immunochemically indistinguishable from the platelet VLA-2 (glycoprotein Ia-IIa) complex.
    Blood. 1989 Apr;73(5):1235-41 PMID: 2930838
  46. Cell attachment properties of collagen type VI and Arg-Gly-Asp dependent binding to its alpha 2(VI) and alpha 3(VI) chains.
    Exp Cell Res. 1989 Apr;181(2):463-74 PMID: 2924798
  47. A laminin-like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction.
    Nature. 1989 Mar 16;338(6212):229-34 PMID: 2922051
  48. Laminin, a multidomain protein. The A chain has a unique globular domain and homology with the basement membrane proteoglycan and the laminin B chains.
    J Biol Chem. 1988 Nov 15;263(32):16536-44 PMID: 3182802
  49. A novel synthetic peptide from the B1 chain of laminin with heparin-binding and cell adhesion-promoting activities.
    J Cell Biol. 1988 Sep;107(3):1253-60 PMID: 3417782
  50. The membrane glycoprotein Ia-IIa (VLA-2) complex mediates the Mg++-dependent adhesion of platelets to collagen.
    J Cell Biol. 1989 May;108(5):1917-24 PMID: 2715183
  51. Laminin and other basement membrane components.
    Annu Rev Cell Biol. 1987;3:57-85 PMID: 3318883
  52. A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody.
    J Biol Chem. 1987 Jul 25;262(21):10376-83 PMID: 3301835
  53. Characterization of the cell surface heterodimer VLA-4 and related peptides.
    J Biol Chem. 1987 Aug 25;262(24):11478-85 PMID: 3497921
  54. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines.
    J Immunol. 1984 Jun;132(6):3011-8 PMID: 6327814
  55. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule.
    Nature. 1984 May 3-9;309(5963):30-3 PMID: 6325925
  56. Monoclonal antibodies against human platelet glycoprotein IIIa.
    Br J Haematol. 1983 Nov;55(3):509-22 PMID: 6227332
  57. Characterization of a novel differentiation antigen complex recognize by a monoclonal antibody (A-1A5): unique activation-specific molecular forms on stimulated T cells.
    J Immunol. 1983 Jul;131(1):334-40 PMID: 6408178
  58. The heparin-binding domain of laminin is responsible for its effects on neurite outgrowth and neuronal survival.
    EMBO J. 1984 Jul;3(7):1463-8 PMID: 6745238
  59. Protease resistance and conformation of laminin.
    Eur J Biochem. 1982 Mar;123(1):63-72 PMID: 7040076
  60. Nerve growth factor, laminin, and fibronectin promote neurite growth in human fetal sensory ganglia cultures.
    J Neurosci Res. 1982;8(2-3):179-93 PMID: 7154111
  61. Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line.
    Cell. 1979 Feb;16(2):277-87 PMID: 88263
  62. Laminin--a glycoprotein from basement membranes.
    J Biol Chem. 1979 Oct 10;254(19):9933-7 PMID: 114518
  63. Cell surface proteoglycan binds mouse mammary epithelial cells to fibronectin and behaves as a receptor for interstitial matrix.
    J Cell Biol. 1988 Feb;106(2):423-30 PMID: 2963012
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.6.2145
Published
1990-06-00
Pages
2145-55
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116116
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.110.6.2145 PMC Full Text View journal details
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