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PMID: 1824871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of nuclear proteins that specifically bind to RNAs containing 5' splice sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 2 ·1991-01-15 ·Pages 320-4

Stolow DT, Berget SM

Abstract

Two polypeptides of 26 and 37 kDa (designated SPP-1 and SPP-2) were identified in in vitro splicing extracts by UV crosslinking to splicing precursor RNAs. Crosslinking of both polypeptides required a functional 5' splice site but was not dependent on sequences at the 3' end of the intron. Centrifugation of extract separated the two polypeptides from major U small nuclear ribonucleoproteins (snRNPs), including U1 snRNPs. Both polypeptides crosslinked to precursor RNAs containing 5' splice sites in the absence of U1 RNA. Complexes containing both polypeptides also contained U1 snRNPs, suggesting that SPP-1 and SPP-2 are a part of the functional spliceosome. We propose that SPP-1 and SPP-2 are factors that participate in the recognition of 5' splice sites.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Binding Sites Humans Introns Kinetics Molecular Sequence Data Nuclear Proteins/metabolism RNA Precursors/genetics,metabolism,radiation effects RNA Splicing Restriction Mapping Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear Transcription, Genetic Ultraviolet Rays
Chemicals
Nuclear Proteins RNA Precursors Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stolow D T
Marrs McClean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
Berget S M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.2.320
Published
1991-01-15
Pages
320-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50802
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.2.320 PMC Full Text View journal details
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