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PMID: 17875746 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Nuclear mRNA export requires specific FG nucleoporins for translocation through the nuclear pore complex.

The Journal of cell biology ·Vol. 178 ·No. 7 ·2007-09-24 ·Pages 1121-32

Terry LJ, Wente SR

Abstract

Trafficking of nucleic acids and large proteins through nuclear pore complexes (NPCs) requires interactions with NPC proteins that harbor FG (phenylalanine-glycine) repeat domains. Specialized transport receptors that recognize cargo and bind FG domains facilitate these interactions. Whether different transport receptors utilize preferential FG domains in intact NPCs is not fully resolved. In this study, we use a large-scale deletion strategy in Saccharomyces cerevisiae to generate a new set of more minimal pore (mmp) mutants that lack specific FG domains. A comparison of messenger RNA (mRNA) export versus protein import reveals unique subsets of mmp mutants with functional defects in specific transport receptors. Thus, multiple functionally independent NPC translocation routes exist for different transport receptors. Our global analysis of the FG domain requirements in mRNA export also finds a requirement for two NPC substructures-one on the nuclear NPC face and one in the NPC central core. These results pinpoint distinct steps in the mRNA export mechanism that regulate NPC translocation efficiency.

MeSH Terms
Active Transport, Cell Nucleus Cell Nucleus/metabolism Mutation/genetics Nuclear Pore/metabolism Nuclear Pore Complex Proteins/chemistry,metabolism Nuclear Proteins/metabolism Nucleocytoplasmic Transport Proteins/metabolism Poly A/metabolism Protein Binding Protein Structure, Tertiary Protein Transport RNA Transport RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism Structure-Activity Relationship Temperature beta Karyopherins/metabolism
Chemicals
MEX67 protein, S cerevisiae NUP57 protein, S cerevisiae Nuclear Pore Complex Proteins Nuclear Proteins Nucleocytoplasmic Transport Proteins RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins beta Karyopherins Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Terry Laura J
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Wente Susan R
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200704174
Published
2007-09-24
Epub
2007-00-17
Pages
1121-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064648
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051219 · United States
NCI NIH HHS · T32 CA009385 · United States
NCI NIH HHS · 5T32-CA009385 · United States
NIGMS NIH HHS · R01-GM51219 · United States
External Links
PubMed source DOI: 10.1083/jcb.200704174 PMC Full Text View journal details
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