Home LiteratureArticle Details
PMID: 9353255 Published · ppublish English Journal Article

The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region.

Genes & development ·Vol. 11 ·No. 21 ·1997-11-01 ·Pages 2857-68

Stutz F, Kantor J, Zhang D, McCarthy T, Neville M, Rosbash M

Abstract

The FG-repeat domain of the yeast Rip1 protein (Rip1p) was identified initially as a possible target for the nuclear export signal (NES) of the HIV-1 Rev protein in a yeast two-hybrid assay. Rip1p is inessential, associated with nuclear pore complexes, and structurally related to the FG-nucleoporin family of pore proteins. It contributes to HIV-1 Rev-mediated RNA export and is also important for the export of heat shock RNAs at 42 degrees C. We show here that Rip1p is essential for the export of heat shock RNAs, and this function is fulfilled by the unique carboxyl terminus of Rip1p with no substantial contribution from the FG-repeat region. Genetic interactions between Rip1p and the RNA export mediator Gle1p are described, which support a role of the carboxyl terminus of Rip1p in poly(A)+ RNA export. Finally, this domain of Rip1p also contributes to Rev-mediated RNA export. The data suggest that Rip1p promotes the nuclear export of different classes of substrates by contributing to optimal pore function.

MeSH Terms
Amino Acid Sequence Cell Nucleus/metabolism DNA Primers Gene Products, rev/biosynthesis,metabolism Genotype HIV-1/genetics Nuclear Envelope/metabolism Nuclear Pore Complex Proteins Nuclear Proteins/metabolism Plasmids Polymerase Chain Reaction RNA, Fungal/metabolism Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins Signal Transduction rev Gene Products, Human Immunodeficiency Virus
Chemicals
DNA Primers Gene Products, rev NUP42 protein, S cerevisiae Nuclear Pore Complex Proteins Nuclear Proteins RNA, Fungal Saccharomyces cerevisiae Proteins rev Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Stutz F
Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA.
Kantor J
Zhang D
McCarthy T
Neville M
Rosbash M
References (52)
52 references, click to expand
  1. A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus.
    Mol Cell Biol. 1996 Dec;16(12):7144-50 PMID: 8943370
  2. Genetic approaches to nuclear pore structure and function.
    Trends Genet. 1995 Jun;11(6):235-41 PMID: 7638906
  3. Cloning by function: an alternative approach for identifying yeast homologs of genes from other organisms.
    Proc Natl Acad Sci U S A. 1990 Sep;87(17):6629-33 PMID: 2204059
  4. The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export.
    Mol Biol Cell. 1996 Oct;7(10):1601-21 PMID: 8898365
  5. Use of a screen for synthetic lethal and multicopy suppressee mutants to identify two new genes involved in morphogenesis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Mar;11(3):1295-305 PMID: 1996092
  6. Pores for thought: nuclear pore complex proteins.
    Trends Cell Biol. 1994 Oct;4(10):357-65 PMID: 14731624
  7. Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p.
    J Cell Biol. 1994 Aug;126(3):619-30 PMID: 8045927
  8. A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p.
    J Cell Biol. 1997 May 19;137(4):797-811 PMID: 9151683
  9. Exploring nuclear pore complex structure and function in molecular detail.
    J Cell Sci Suppl. 1995;19:1-11 PMID: 8655639
  10. Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins.
    Cell. 1995 Aug 11;82(3):485-94 PMID: 7634337
  11. Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis.
    Mol Cell Biol. 1987 May;7(5):1602-11 PMID: 3299050
  12. A pre-mRNA-binding protein accompanies the RNA from the gene through the nuclear pores and into polysomes.
    Cell. 1996 Jan 26;84(2):253-64 PMID: 8565071
  13. Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast.
    Cell. 1995 Aug 11;82(3):495-506 PMID: 7634338
  14. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.
    Yeast. 1994 Dec;10(13):1793-808 PMID: 7747518
  15. A role for the M9 transport signal of hnRNP A1 in mRNA nuclear export.
    J Cell Biol. 1997 Apr 7;137(1):27-35 PMID: 9105034
  16. Regulation of mRNA export in response to stress in Saccharomyces cerevisiae.
    Genes Dev. 1996 Jul 1;10(13):1608-20 PMID: 8682292
  17. The heat-shock proteins.
    Annu Rev Genet. 1988;22:631-77 PMID: 2853609
  18. The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export.
    Curr Biol. 1997 Oct 1;7(10):767-75 PMID: 9368759
  19. Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev.
    Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2936-40 PMID: 8610146
  20. Nuclear export of different classes of RNA is mediated by specific factors.
    J Cell Biol. 1994 Mar;124(5):627-35 PMID: 7509815
  21. Classical mutagenesis techniques.
    Methods Enzymol. 1991;194:273-81 PMID: 2005792
  22. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
    Cell. 1995 Aug 11;82(3):475-83 PMID: 7543368
  23. Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p.
    Mol Biol Cell. 1996 Jun;7(6):917-34 PMID: 8816998
  24. An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing.
    Genes Dev. 1993 Mar;7(3):419-28 PMID: 8449403
  25. A GTPase controlling nuclear trafficking: running the right way or walking RANdomly?
    Cell. 1996 Oct 4;87(1):1-4 PMID: 8858141
  26. HIV Rev uses a conserved cellular protein export pathway for the nucleocytoplasmic transport of viral RNAs.
    Curr Biol. 1996 Jul 1;6(7):848-54 PMID: 8805303
  27. NUP82 is an essential yeast nucleoporin required for poly(A)+ RNA export.
    J Cell Biol. 1995 Sep;130(6):1275-81 PMID: 7559751
  28. Protein sequence requirements for function of the human T-cell leukemia virus type 1 Rex nuclear export signal delineated by a novel in vivo randomization-selection assay.
    Mol Cell Biol. 1996 Aug;16(8):4207-14 PMID: 8754820
  29. Nucleocytoplasmic transport of macromolecules.
    Microbiol Mol Biol Rev. 1997 Jun;61(2):193-211 PMID: 9184010
  30. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores.
    Cell. 1996 Jan 26;84(2):265-75 PMID: 8565072
  31. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
    Cell. 1989 May 19;57(4):573-83 PMID: 2655924
  32. Toward the molecular dissection of protein import into nuclei.
    Curr Opin Cell Biol. 1996 Jun;8(3):397-406 PMID: 8743893
  33. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  34. mRNA splicing efficiency in yeast and the contribution of nonconserved sequences.
    Cell. 1985 May;41(1):119-26 PMID: 3888403
  35. Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in xenopus oocytes.
    Cell. 1990 Feb 23;60(4):619-28 PMID: 2137374
  36. The nuclear pore complex.
    Annu Rev Biochem. 1995;64:865-96 PMID: 7574503
  37. Identification of a signal for rapid export of proteins from the nucleus.
    Cell. 1995 Aug 11;82(3):463-73 PMID: 7634336
  38. A human nucleoporin-like protein that specifically interacts with HIV Rev.
    Nature. 1995 Aug 10;376(6540):530-3 PMID: 7637788
  39. Mechanisms of nuclear protein import.
    Curr Opin Cell Biol. 1995 Jun;7(3):310-8 PMID: 7662359
  40. A second post-transcriptional trans-activator gene required for HTLV-III replication.
    Nature. 1986 May 22-28;321(6068):412-7 PMID: 3012355
  41. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export.
    Genes Dev. 1996 May 15;10(10):1233-46 PMID: 8675010
  42. Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.
    EMBO J. 1997 Jun 2;16(11):3256-71 PMID: 9214641
  43. A cap-binding protein complex mediating U snRNA export.
    Nature. 1995 Aug 24;376(6542):709-12 PMID: 7651522
  44. A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
    Cell. 1995 Nov 3;83(3):415-22 PMID: 8521471
  45. The HIV-1 Rev protein: prototype of a novel class of eukaryotic post-transcriptional regulators.
    Trends Biochem Sci. 1991 Sep;16(9):346-50 PMID: 1949157
  46. Nucleocytoplasmic transport.
    Science. 1996 Mar 15;271(5255):1513-8 PMID: 8599106
  47. Nucleocytoplasmic transport: signals, mechanisms and regulation.
    Nature. 1997 Apr 24;386(6627):779-87 PMID: 9126736
  48. An RNA-export mediator with an essential nuclear export signal.
    Nature. 1996 Sep 26;383(6598):357-60 PMID: 8848052
  49. A nuclear cap-binding complex binds Balbiani ring pre-mRNA cotranscriptionally and accompanies the ribonucleoprotein particle during nuclear export.
    J Cell Biol. 1996 Apr;133(1):5-14 PMID: 8601613
  50. A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p.
    J Cell Biol. 1995 Sep;130(6):1263-73 PMID: 7559750
  51. A functional interaction between Rev and yeast pre-mRNA is related to splicing complex formation.
    EMBO J. 1994 Sep 1;13(17):4096-104 PMID: 8076605
  52. Nuclear export signals and the fast track to the cytoplasm.
    Cell. 1995 Aug 11;82(3):341-4 PMID: 7634321
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.11.21.2857
Published
1997-11-01
Pages
2857-68
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316647
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.11.21.2857 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2