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PMID: 12065398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural basis for the interaction between NTF2 and nucleoporin FxFG repeats.

The EMBO journal ·Vol. 21 ·No. 12 ·2002-06-17 ·Pages 2843-53

Bayliss R, Leung SW, Baker RP, Quimby BB, Corbett AH, Stewart M

Abstract

Interactions with nucleoporins containing FxFG-repeat cores are crucial for the nuclear import of RanGDP mediated by nuclear transport factor 2 (NTF2). We describe here the 1.9 A resolution crystal structure of yeast NTF2-N77Y bound to a FxFG-nucleoporin core, which provides a basis for understanding this interaction and its role in nuclear trafficking. The two identical FxFG binding sites on the dimeric molecule are formed by residues from each chain of NTF2. Engineered mutants at the interaction interface reduce the binding of NTF2 to nuclear pores and cause reduced growth rates and Ran mislocalization when substituted for the wild-type protein in yeast. Comparison with the crystal structure of FG-nucleoporin cores bound to importin-beta and TAP/p15 identified a number of common features of their binding sites. The structure of the binding interfaces on these transport factors provides a rationale for the specificity of their interactions with nucleoporins that, combined with their weak binding constants, facilitates rapid translocation through NPCs during nuclear trafficking.

MeSH Terms
Active Transport, Cell Nucleus/physiology Amino Acid Sequence Animals Binding Sites Carrier Proteins/chemistry,genetics,metabolism Crystallography, X-Ray Dimerization Fungal Proteins/chemistry,genetics,metabolism Models, Molecular Molecular Sequence Data Nuclear Pore Complex Proteins/chemistry,metabolism Nucleocytoplasmic Transport Proteins/chemistry,genetics,metabolism Peptides/chemistry,metabolism Protein Binding Protein Structure, Tertiary Rats Sequence Alignment
Chemicals
Carrier Proteins Fungal Proteins Nuclear Pore Complex Proteins Nucleocytoplasmic Transport Proteins Nutf2 protein, rat Peptides
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bayliss Richard
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Leung Sara W
Baker Rosanna P
Quimby B Booth
Corbett Anita H
Stewart Murray
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf305
Published
2002-06-17
Pages
2843-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC126060
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdf305 PMC Full Text View journal details
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