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PMID: 17591921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A role for Rab5 in structuring the endoplasmic reticulum.

The Journal of cell biology ·Vol. 178 ·No. 1 ·2007-07-02 ·Pages 43-56

Audhya A, Desai A, Oegema K

Abstract

The endoplasmic reticulum (ER) is a contiguous network of interconnected membrane sheets and tubules. The ER is differentiated into distinct domains, including the peripheral ER and nuclear envelope. Inhibition of two ER proteins, Rtn4a and DP1/NogoA, was previously shown to inhibit the formation of ER tubules in vitro. We show that the formation of ER tubules in vitro also requires a Rab family GTPase. Characterization of the 29 Caenorhabditis elegans Rab GTPases reveals that depletion of RAB-5 phenocopies the defects in peripheral ER structure that result from depletion of RET-1 and YOP-1, the C. elegans homologues of Rtn4a and DP1/NogoA. Perturbation of endocytosis by other means did not affect ER structure; the role of RAB-5 in ER morphology is thus independent of its well-studied requirement for endocytosis. RAB-5 and YOP-1/RET-1 also control the kinetics of nuclear envelope disassembly, which suggests an important role for the morphology of the peripheral ER in this process.

MeSH Terms
Animals Caenorhabditis elegans/genetics,metabolism Cell Extracts/chemistry Embryo, Nonmammalian Endoplasmic Reticulum/metabolism Female Green Fluorescent Proteins/metabolism Histones/genetics,metabolism Models, Biological Ovum/chemistry RNA Interference RNA, Helminth Recombinant Fusion Proteins/metabolism Xenopus laevis rab5 GTP-Binding Proteins/metabolism
Chemicals
Cell Extracts Histones RNA, Helminth Recombinant Fusion Proteins Green Fluorescent Proteins rab5 GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Audhya Anjon
Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA. aaudhya@ucsd.edu
Desai Arshad
Oegema Karen
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200701139
Email
aaudhya@ucsd.edu
Published
2007-07-02
Epub
2007-00-25
Pages
43-56
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064419
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.200701139 PMC Full Text View journal details
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