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PMID: 12937276 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo.

Molecular biology of the cell ·Vol. 14 ·No. 12 ·2003-12-00 ·Pages 5104-15

Galy V, Mattaj IW, Askjaer P

Abstract

Nuclear pore complexes (NPCs) span the nuclear envelope and mediate communication between the nucleus and the cytoplasm. To obtain insight into the structure and function of NPCs of multicellular organisms, we have initiated an extensive analysis of Caenorhabditis elegans nucleoporins. Of 20 assigned C. elegans nucleoporin genes, 17 were found to be essential for embryonic development either alone or in combination. In several cases, depletion of nucleoporins by RNAi caused severe defects in nuclear appearance. More specifically, the C. elegans homologs of vertebrate Nup93 and Nup205 were each found to be required for normal NPC distribution in the nuclear envelope in vivo. Depletion of Nup93 or Nup205 caused a failure in nuclear exclusion of nonnuclear macromolecules of approximately 70 kDa without preventing active nuclear protein import or the assembly of the nuclear envelope. The defects in NPC exclusion were accompanied by abnormal chromatin condensation and early embryonic arrest. Thus, the contribution to NPC structure of Nup93 and Nup205 is essential for establishment of normal NPC function and for cell viability.

MeSH Terms
Animals Animals, Genetically Modified/embryology,genetics,metabolism Caenorhabditis elegans/embryology,genetics,metabolism Fluorescent Antibody Technique Nuclear Envelope/metabolism Nuclear Pore/genetics,metabolism Nuclear Pore Complex Proteins/drug effects,genetics,metabolism RNA, Small Interfering/pharmacology Xenopus Proteins/genetics,metabolism
Chemicals
Nuclear Pore Complex Proteins Nup93 protein, Xenopus RNA, Small Interfering Xenopus Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Galy Vincent
European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
Mattaj Iain W
Askjaer Peter
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e03-04-0237
Published
2003-12-00
Epub
2003-00-22
Pages
5104-15
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC284812
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e03-04-0237 PMC Full Text View journal details
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