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PMID: 1741293 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Unusual DNA binding characteristics of an in vitro translation product of the CCAAT binding protein mYB-1.

Nucleic acids research ·Vol. 20 ·No. 3 ·1992-02-11 ·Pages 601-6

Gai XX, Lipson KE, Prystowsky MB

Abstract

We have isolated a cDNA that encodes the murine CCAAT-binding protein mYB-1. The deduced amino acid sequence shows 95% identity with its presumed human homologue (hYB-1A) which was originally isolated as a protein that binds to the Y box of MHC class II genes. In vitro translated mYB-1 binds to CCAAT boxes of the MHCIIE alpha, HSVTK and mouse PCNA promoters but not to alpha-globin or human thymidine kinase CCAAT boxes. Interestingly, complexes formed between the in vitro translated protein and the various CCAAT boxes display the property of being competed more efficiently with self competitor DNA, regardless of the CCAAT box initially used as a probe. A similar phenomenon was observed in a cell extract of Con-A stimulated murine splenocytes when the same competition assays were performed. These results may reflect the generation of multiple forms of a particular CCAAT-binding protein, such as mYB-1, that display distinct, yet overlapping, DNA binding specificities.

MeSH Terms
Animals Base Sequence Blotting, Southern CCAAT-Enhancer-Binding Proteins Cells, Cultured DNA/metabolism DNA-Binding Proteins/metabolism Genes, MHC Class II/genetics Humans Mice Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Oligodeoxyribonucleotides/genetics,metabolism Regulatory Sequences, Nucleic Acid/genetics Transcription Factors Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Oligodeoxyribonucleotides Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gai X X
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Lipson K E
Prystowsky M B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.3.601
Published
1992-02-11
Pages
601-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310429
Subset
IM
Grants
NIGMS NIH HHS · GM-41226 · United States
Databases
GENBANK
X57621
External Links
PubMed source DOI: 10.1093/nar/20.3.601 PMC Full Text View journal details
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