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PMID: 17355986 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

ElrA binding to the 3'UTR of cyclin E1 mRNA requires polyadenylation elements.

Nucleic acids research ·Vol. 35 ·No. 7 ·2007-00-00 ·Pages 2167-76

Slevin MK, Gourronc F, Hartley RS

Abstract

The early cell divisions of Xenopus laevis and other metazoan embryos occur in the presence of constitutively high levels of the cell cycle regulator cyclin E1. Upon completion of the 12th cell division, a time at which many maternal proteins are downregulated by deadenylation and destabilization of their encoding mRNAs, maternal cyclin E1 protein is downregulated while its mRNA is polyadenylated and stable. We report here that stable polyadenylation of cyclin E1 mRNA requires three cis-acting elements in the 3' untranslated region; the nuclear polyadenylation sequence, a contiguous cytoplasmic polyadenylation element and an upstream AU-rich element. ElrA, the Xenopus homolog of HuR and a member of the ELAV gene family binds the cyclin E1 3'UTR with high affinity. Deletion of these elements dramatically reduces the affinity of ElrA for the cyclin E1 3'UTR, abolishes polyadenylation and destabilizes the mRNA. Together, these findings provide compelling evidence that ElrA functions in polyadenylation and stabilization of cyclin E1 mRNA via binding these elements.

MeSH Terms
3' Untranslated Regions/chemistry,metabolism Animals Binding Sites Cyclin E/genetics Polyadenylation Regulatory Sequences, Ribonucleic Acid Ribonucleoproteins/metabolism Xenopus Proteins/metabolism Xenopus laevis
Chemicals
3' Untranslated Regions Cyclin E ELAVL1 protein, Xenopus Regulatory Sequences, Ribonucleic Acid Ribonucleoproteins Xenopus Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Slevin Michael K
Molecular and Cellular Biology Program, University of Iowa, Iowa City, IA 52242, USA.
Gourronc Francoise
Hartley Rebecca S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkm084
Published
2007-00-00
Epub
2007-00-13
Pages
2167-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1874641
Subset
IM
Grants
NCI NIH HHS · R01 CA095898 · United States
NCI NIH HHS · CA095898-01A1 · United States
External Links
PubMed source DOI: 10.1093/nar/gkm084 PMC Full Text View journal details
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