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PMID: 9343402 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The 36-kilodalton embryonic-type cytoplasmic polyadenylation element-binding protein in Xenopus laevis is ElrA, a member of the ELAV family of RNA-binding proteins.

Molecular and cellular biology ·Vol. 17 ·No. 11 ·1997-11-00 ·Pages 6402-9

Wu L, Good PJ, Richter JD

Abstract

The translational activation of several maternal mRNAs in Xenopus laevis is dependent on cytoplasmic poly(A) elongation. Messages harboring the UUUUUAU-type cytoplasmic polyadenylation element (CPE) in their 3' untranslated regions (UTRs) undergo polyadenylation and translation during oocyte maturation. This CPE is bound by the protein CPEB, which is essential for polyadenylation. mRNAs that have the poly(U)12-27 embryonic-type CPE (eCPE) in their 3' UTRs undergo polyadenylation and translation during the early cleavage and blastula stages. A 36-kDa eCPE-binding protein in oocytes and embryos has been identified by UV cross-linking. We now report that this 36-kDa protein is ElrA, a member of the ELAV family of RNA-binding proteins. The proteins are identical in size, antibody directed against ElrA immunoprecipitates the 36-kDa protein, and the two proteins have the same RNA binding specificity in vitro. C12 and activin receptor mRNAs, both of which contain eCPEs, are detected in immunoprecipitated ElrA-mRNP complexes from eggs and embryos. In addition, this in vivo interaction requires the eCPE. Although a number of experiments failed to define a role for ElrA in cytoplasmic polyadenylation, the expression of a dominant negative ElrA protein in embryos results in an exogastrulation phenotype. The possible functions of ElrA in gastrulation are discussed.

MeSH Terms
Animals Cytoplasm/metabolism Gastrula Mutation Poly A Protein Binding RNA Processing, Post-Transcriptional RNA, Messenger/metabolism RNA-Binding Proteins/metabolism Regulatory Sequences, Nucleic Acid Sequence Deletion Transcription Factors/metabolism Xenopus Proteins Xenopus laevis/embryology mRNA Cleavage and Polyadenylation Factors
Chemicals
Cpeb1 protein, Xenopus RNA, Messenger RNA-Binding Proteins Transcription Factors Xenopus Proteins mRNA Cleavage and Polyadenylation Factors Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wu L
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester 01655, USA.
Good P J
Richter J D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.11.6402
Published
1997-11-00
Pages
6402-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232492
Subset
IM
Grants
NCI NIH HHS · CA40189 · United States
NIGMS NIH HHS · GM17932 · United States
NIGMS NIH HHS · GM46779 · United States
External Links
PubMed source DOI: 10.1128/MCB.17.11.6402 PMC Full Text View journal details
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