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PMID: 9278474 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA.

Nucleic acids research ·Vol. 25 ·No. 18 ·1997-09-15 ·Pages 3564-9

Ma WJ, Chung S, Furneaux H

Abstract

The Elav-like proteins are specific mRNA binding proteins which are required for cellular differentiation. They contain three characteristic RNP2/RNP1-type RNA binding motifs. Previously we have shown that the first and second RNA binding domains bind to AU-rich elements in the 3'-UTR of mRNA. In this paper we show that the Elav-like proteins exhibit poly(A) binding activity. This activity is distinct from poly(A) binding activities that have been previously described. The Elav-like proteins specifically bind to long chain poly(A) tails. We have shown that the third RNA binding domain encompasses this poly(A) binding activity. Using poly(A)-Sepharose beads in a 'sandwich' assay we have shown that the Elav-like proteins can bind simultaneously to the AU-rich element and to the poly(A) tail.

MeSH Terms
Base Sequence Binding Sites/genetics ELAV Proteins Molecular Sequence Data RNA, Messenger/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Ribonucleoproteins/genetics,metabolism Sequence Analysis
Chemicals
ELAV Proteins RNA, Messenger RNA-Binding Proteins Ribonucleoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ma W J
Program in Molecular Pharmacology and Therapeutics, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021 USA.
Chung S
Furneaux H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.18.3564
Published
1997-09-15
Pages
3564-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146929
Subset
IM
Grants
NINDS NIH HHS · NS29682 · United States
NCI NIH HHS · P30-CA08748 · United States
External Links
PubMed source DOI: 10.1093/nar/25.18.3564 PMC Full Text View journal details
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