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PMID: 17158930 Published · ppublish English Journal Article Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Distinctive signatures of histone methylation in transcribed coding and noncoding human beta-globin sequences.

Molecular and cellular biology ·Vol. 27 ·No. 4 ·2007-02-00 ·Pages 1271-9

Kim A, Kiefer CM, Dean A

Abstract

The establishment of epigenetic marks, such as methylation on histone tails, is mechanistically linked to RNA polymerase II within active genes. To explore the interplay between these modifications in transcribed noncoding as well as coding sequences, we analyzed epigenetic modification and chromatin structure at high resolution across 300 kb of human chromosome 11, including the beta-globin locus which is extensively transcribed in intergenic regions. Monomethylated H3K4, K9, and K36 were broadly distributed, while hypermethylated forms appeared to different extents across the region in a manner reflecting transcriptional activity. The trimethylation of H3K4 and H3K9 correlated within the most highly transcribed sequences. The H3K36me3 mark was more broadly detected in transcribed coding and noncoding sequences, suggesting that K36me3 is a stable mark on sequences transcribed at any level. Most epigenetic and chromatin structural features did not undergo transitions at the presumed borders of the globin domain where the insulator factor CTCF interacts, raising questions about the function of the borders.

MeSH Terms
Acetylation Base Sequence Chromatin/metabolism DNA, Intergenic Deoxyribonuclease I/metabolism Epigenesis, Genetic Globins/chemistry,genetics,metabolism Histones/metabolism Humans K562 Cells Lysine/metabolism Methylation Open Reading Frames/genetics Protein Structure, Tertiary RNA Polymerase II/metabolism RNA, Messenger/genetics,metabolism Transcription, Genetic
Chemicals
Chromatin DNA, Intergenic Histones RNA, Messenger Globins RNA Polymerase II Deoxyribonuclease I Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kim AeRi
Department of Molecular Biology, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea. kimaeri@pusan.ac.kr
Kiefer Christine M
Dean Ann
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.01684-06
Email
kimaeri@pusan.ac.kr
Published
2007-02-00
Epub
2006-00-11
Pages
1271-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1800709
Subset
IM
Grants
Intramural NIH HHS · United States
External Links
PubMed source DOI: 10.1128/MCB.01684-06 PMC Full Text View journal details
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