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PMID: 12612070 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA polymerase II accumulation in the promoter-proximal region of the dihydrofolate reductase and gamma-actin genes.

Molecular and cellular biology ·Vol. 23 ·No. 6 ·2003-03-00 ·Pages 1961-7

Cheng C, Sharp PA

Abstract

The carboxyl-terminal domain (CTD) of RNA polymerase II (Pol II) can be phosphorylated at serine 2 (Ser-2) and serine 5 (Ser-5) of the CTD heptad repeat YSPTSPS, and this phosphorylation is important in coupling transcription to RNA processing, including 5' capping, splicing, and polyadenylation. The mammalian endogenous dihydrofolate reductase and gamma-actin genes have been used to study the association of Pol II with different regions of transcribed genes (promoter-proximal compared to distal regions) and the phosphorylation status of its CTD. For both genes, Pol II is more concentrated in the promoter-proximal regions than in the interior regions. Moreover, different phosphorylation forms of Pol II are associated with distinct regions. Ser-5 phosphorylation of Pol II is concentrated near the promoter, while Ser-2 phosphorylation is observed throughout the gene. These results suggest that the accumulation of paused Pol II in promoter-proximal regions may be a common feature of gene regulation in mammalian cells.

MeSH Terms
Actins/genetics Chromatin/metabolism,ultrastructure HeLa Cells Humans Neoplasm Proteins/metabolism Phosphorylation Promoter Regions, Genetic Protein Processing, Post-Translational Protein Structure, Tertiary RNA Polymerase II/chemistry,metabolism Tetrahydrofolate Dehydrogenase/genetics Transcription, Genetic
Chemicals
Actins Chromatin Neoplasm Proteins Tetrahydrofolate Dehydrogenase RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cheng Chonghui
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA.
Sharp Phillip A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.6.1961-1967.2003
Published
2003-03-00
Pages
1961-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC149466
Subset
IM
Grants
NCI NIH HHS · P01 CA042063 · United States
NCI NIH HHS · P30 CA014051 · United States
NCI NIH HHS · P01 CA 42063 · United States
NCI NIH HHS · P30 CA 14051 · United States
External Links
PubMed source DOI: 10.1128/MCB.23.6.1961-1967.2003 PMC Full Text View journal details
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