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PMID: 1714321 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Isolation and characterization of a soybean hsp70 gene.

Plant molecular biology ·Vol. 16 ·No. 4 ·1991-04-00 ·Pages 671-83

Roberts JK, Key JL

Abstract

Soybean, like many other organisms, responds to an increase in growth temperature by producing a set of new proteins, heat shock proteins. The heat shock proteins have been classified into several categories according to their molecular weight. Data are presented on the isolation, sequence characterization, and expression of a 70 kDa heat shock protein gene from soybean. A cDNA clone was isolated using a Drosophila hsp70 clone as a heterologous probe, and the cDNA was used for isolation of the soybean gene corresponding to the cDNA. The structure of this soybean is very similar to the hsp70 genes from other organisms. It has several sequences in the 5' untranscribed region that are similar to the well characterized heat shock consensus element found in other organisms. These heat shock consensus elements have the expected position relative to the start of transcription. Unlike hsp70-like genes previously isolated from other plants, this gene does not have an intron. This protein shows high amino acid sequence similarity to other hsp70 proteins from such diverse organisms as Drosophila, rat, and Xenopus. This soybean gene is only expressed during heat shock. In addition to the hsp70 gene isolated here, there is evidence for many other hsp70-like genes in soybean.

Related Genes
hsp70
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA/genetics,isolation & purification Drosophila melanogaster/genetics Gene Library Genes, Plant Heat-Shock Proteins/genetics Molecular Sequence Data Molecular Weight Poly A/genetics Protein Biosynthesis RNA/genetics RNA, Messenger Sequence Homology, Nucleic Acid Soybeans/genetics Transcription, Genetic
Chemicals
Heat-Shock Proteins RNA, Messenger Poly A RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roberts J K
Department of Biochemistry, University of Georgia, Athens 30602.
Key J L
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00023431
Published
1991-04-00
Pages
671-83
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00023431 View journal details
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