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PMID: 1709485 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The promoter and enhancer of the inactive chicken beta-globin gene contains precisely positioned nucleosomes.

Nucleic acids research ·Vol. 19 ·No. 6 ·1991-03-25 ·Pages 1219-26

Buckle R, Balmer M, Yenidunya A, Allan J

Abstract

Core histone octamers reconstituted in vitro onto DNA fragments containing the chicken beta-globin gene promoter are precisely positioned with respect to the underlying DNA sequence [1]. Here we show that this is also true of the chicken beta-globin gene enhancer. These nucleosome binding sites are also employed within transfected COS cell nuclei, where the chicken beta-globin gene is transcriptionally inactive. Similar results were found in vivo, where positioned nucleosomes were detected over the inactive beta-globin promoter in chicken brain cells and 5-day red blood cells, and over the inactive beta-globin enhancer in brain cells. In contrast, the promoter and enhancer regions were found to be nucleosome-free in 15-day erythrocytes where the beta-globin gene is active. We argue that these results suggest a role for positioned nucleosomes in the regulation of the transcription of the chicken beta-globin gene.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line Chickens Electrophoresis, Agar Gel Enhancer Elements, Genetic Exodeoxyribonucleases Globins/genetics Molecular Sequence Data Nucleosomes/metabolism Plasmids Promoter Regions, Genetic RNA/isolation & purification Restriction Mapping Transcription, Genetic Transfection
Chemicals
Nucleosomes RNA Globins Exodeoxyribonucleases exodeoxyribonuclease III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Buckle R
Department of Biophysics, Cell and Molecular Biology, King's College, London, UK.
Balmer M
Yenidunya A
Allan J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.6.1219
Published
1991-03-25
Pages
1219-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333846
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.6.1219 PMC Full Text View journal details
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