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PMID: 2835669 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Role for DNA replication in beta-globin gene activation.

Molecular and cellular biology ·Vol. 8 ·No. 3 ·1988-03-00 ·Pages 1301-8

Enver T, Brewer AC, Patient RK

Abstract

Transcriptional activation of the Xenopus laevis beta-globin gene requires the synergistic action of the simian virus 40 enhancer and DNA replication in DEAE-dextran-mediated HeLa cell transfections. Replication does not act through covalent modification of the template, since its requirement was not obviated by the prior replication of the transfected DNA in eucaryotic cells. Transfection of DNA over a 100-fold range demonstrates that replication does not contribute to gene activation simply increasing template copy number. Furthermore, in cotransfections of replicating and nonreplicating constructs, only replicating templates were transcribed. Replication is not simply a requirement of chromatin assembly, since even unreplicated templates generated nucleosomal ladders. Stimulation of beta-globin transcription by DNA replication, though less marked, was also observed in calcium phosphate transfections. We interpret these results as revealing a dynamic role for replication in gene activation.

MeSH Terms
Animals Chromatin/physiology DNA Replication DNA Restriction Enzymes Enhancer Elements, Genetic Gene Expression Regulation Genes Globins/genetics HeLa Cells Humans Simian virus 40/genetics Templates, Genetic Transcription, Genetic Transcriptional Activation Transfection Xenopus laevis
Chemicals
Chromatin Globins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Enver T
Department of Biophysics, Cell and Molecular Biology, King's College London, United Kingdom.
Brewer A C
Patient R K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.3.1301-1308.1988
Published
1988-03-00
Pages
1301-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363276
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/mcb.8.3.1301-1308.1988 PMC Full Text View journal details
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