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PMID: 1708722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Properties of promoter regions of mdg1 Drosophila retrotransposon indicate that it belongs to a specific class of promoters.

The EMBO journal ·Vol. 10 ·No. 5 ·1991-05-00 ·Pages 1169-77

Arkhipova IR, Ilyin YV

Abstract

A sequence 30 bp downstream from the start site of the Drosophila melanogaster retrotransposon mdg1 is shown to be responsible for correct and precise initiation of mdg1 RNA synthesis in combination with the RNA start-site sequence TCAGTT. A sequence-specific DNA binding protein is demonstrated to interact with the +30 sequence, and the efficient binding of this factor is necessary for in vivo transcriptional activity of the plasmid constructs containing mdg1 promoter fragments. The nucleotides -8/+34 of mdg1 represent a minimal promoter which is able to provide correct initiation of transcription by RNA polymerase II at basal levels. A comparison with properties of some other retrotransposable elements and several developmentally regulated cellular genes allows us to conclude that together they form a specific class of RNA polymerase II promoter. This promoter class characteristically lacks upstream sequences necessary for transcription initiation, such as TATA boxes, but requires a specific downstream promoter element within 40 bp downstream of the RNA start site. The level of transcription can, however, be modulated by upstream regulatory elements. The identified sequence-specific downstream initiation factor may be responsible for transcription initiation on promoters of some genes which belong to this class.

MeSH Terms
Animals Base Sequence Binding Sites Blotting, Northern DNA Transposable Elements DNA-Binding Proteins/biosynthesis,genetics Drosophila melanogaster/genetics Molecular Sequence Data Promoter Regions, Genetic RNA/biosynthesis RNA Polymerase II/metabolism TATA Box Transcription, Genetic
Chemicals
DNA Transposable Elements DNA-Binding Proteins RNA RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Arkhipova I R
V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow.
Ilyin Y V
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-05-00
Pages
1169-77
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452770
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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