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PMID: 3099170 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Delimiting regulatory sequences of the Drosophila melanogaster Ddc gene.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4548-57

Hirsh J, Morgan BA, Scholnick SB

Abstract

We delimited sequences necessary for in vivo expression of the Drosophila melanogaster dopa decarboxylase gene Ddc. The expression of in vitro-altered genes was assayed following germ line integration via P-element vectors. Sequences between -209 and -24 were necessary for normally regulated expression, although genes lacking these sequences could be expressed at 10 to 50% of wild-type levels at specific developmental times. These genes showed components of normal developmental expression, which suggests that they retain some regulatory elements. All Ddc genes lacking the normal immediate 5'-flanking sequences were grossly deficient in larval central nervous system expression. Thus, this upstream region must contain at least one element necessary for this expression. A mutated Ddc gene without a normal TATA boxlike sequence used the normal RNA start points, indicating that this sequences is not required for start point specificity.

MeSH Terms
Alcohol Dehydrogenase/genetics Animals Aromatic-L-Amino-Acid Decarboxylases/genetics Base Sequence Chromosome Deletion Dopa Decarboxylase/genetics Drosophila melanogaster/enzymology,genetics Drug Resistance Ethanol/pharmacology Genes Genes, Regulator Genetic Vectors
Chemicals
Ethanol Alcohol Dehydrogenase Dopa Decarboxylase Aromatic-L-Amino-Acid Decarboxylases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hirsh J
Morgan B A
Scholnick S B
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39 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.12.4548-4557.1986
Published
1986-12-00
Pages
4548-57
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367239
Subset
IM
Grants
NIGMS NIH HHS · GM27318 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.12.4548-4557.1986 PMC Full Text View journal details
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