Home LiteratureArticle Details
PMID: 6086012 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster.

Molecular and cellular biology ·Vol. 1 ·No. 6 ·1981-06-00 ·Pages 475-85

Hirsh J, Davidson N

Abstract

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.

MeSH Terms
Animals Aromatic-L-Amino-Acid Decarboxylases/genetics Chromosome Mapping Cloning, Molecular DNA/genetics,ultrastructure Dopa Decarboxylase/genetics Drosophila melanogaster/enzymology,genetics Genes Microscopy, Electron RNA/genetics RNA, Complementary
Chemicals
RNA, Complementary RNA DNA Dopa Decarboxylase Aromatic-L-Amino-Acid Decarboxylases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hirsh J
Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115.
Davidson N
References (36)
36 references, click to expand
  1. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  2. Replication in polytene chromosomes.
    Results Probl Cell Differ. 1972;4:59-85 PMID: 4198832
  3. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  6. Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA.
    Anal Biochem. 1976 May 7;72:413-27 PMID: 782287
  7. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  8. Ecdysone-stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Assay by in situ hybridization.
    Chromosoma. 1976 Oct 12;58(1):87-99 PMID: 826375
  9. The genetics of dopa decarboxylase in Drosophila melanogaster. I. Isolation and characterization of deficiencies that delete the dopa-decarboxylase-dosage-sensitive region and the alpha-methyl-dopa-hypersensitive locus.
    Genetics. 1976 Oct;84(2):267-85 PMID: 826447
  10. The genetics of dopa decarboxylase in Drosophila melanogaster. II. Isolation and characterization of dopa-decarboxylase-deficient mutants and their relationship to the alpha-methyl-dopa-hypersensitive mutants.
    Genetics. 1976 Oct;84(2):287-310 PMID: 826448
  11. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent.
    J Immunol. 1976 Nov;117(5 Pt 1):1482-90 PMID: 187693
  12. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  13. Highly repeated DNA in Drosophila melanogaster.
    J Mol Biol. 1977 May 5;112(1):31-47 PMID: 407366
  14. Ecdysone titers during postembryonic development of Drosophila melanogaster.
    Dev Biol. 1977 Oct 1;60(1):310-7 PMID: 409638
  15. The rabbit beta-globin gene contains a large large insert in the coding sequence.
    Cell. 1977 Dec;12(4):1097-108 PMID: 597859
  16. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  17. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  18. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  19. The use of R-looping for structural gene identification and mRNA purification.
    Nucleic Acids Res. 1979 Jun 11;6(7):2483-97 PMID: 379820
  20. Induction of translatable mRNA for dopa decarboxylase in Drosophila: an early response to ecdysterone.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4175-9 PMID: 6776524
  21. The appearance of dopa decarboxylase activity in imaginal discs of Sarcophaga bullata, undergoing development in vitro.
    Dev Biol. 1974 Jun;38(2):271-84 PMID: 4831109
  22. The selection for mutants in Drosophila melanogaster hypersensitive to alpha-methyl dopa, a dopa decarboxylase inhibitor.
    Mol Gen Genet. 1974 May 21;130(2):127-41 PMID: 4209419
  23. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
    Cell. 1974 Dec;3(4):315-25 PMID: 4216403
  24. Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
    Cell. 1975 Apr;4(4):367-78 PMID: 47272
  25. Translation of mRNA for 3,4-dihydroxyphenylalanine decarboxylase isolated from epidermis tissue of Calliphora vicina R. -D. in a heterologous system. Dependence of mRNA concentration on the insect steroid hormone ecdysone.
    Eur J Biochem. 1975 Feb 3;51(1):305-16 PMID: 1122915
  26. Improved methods for the formation and stabilization of R-loops.
    Nucleic Acids Res. 1979 Jun 11;6(7):2499-317 PMID: 379821
  27. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  28. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  29. Electron microscopic analysis of the banding pattern in the salivary gland chromosomes of Drosophila melanogaster: divisions 37, 38 and 39 of 2L.
    Hereditas. 1979;91(1):5-18 PMID: 116992
  30. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  31. Developmental relationship between dopa decarboxylase, dopamine acetyltransferase, and ecdysone in Drosophila.
    Dev Biol. 1980 Dec;80(2):379-87 PMID: 6778749
  32. The response of dopa decarboxylase activity to variations in gene dosage in Drosophila: a possible location of the structural gene.
    Genetics. 1975 Jan;79(1):45-54 PMID: 1126620
  33. Two-dimensional gel patterns of protein synthesis before and after fertilization of sea urchin eggs.
    Dev Biol. 1976 Sep;52(2):310-7 PMID: 12194440
  34. The relative mutabilities of DNA in regions of the X chromosome of Drosophila melanogaster.
    Genetics. 1965 Sep;52(3):665-81 PMID: 5863912
  35. Liquid cation exchange--a basis for sensitive radiometric assays for aromatic amino acid decarboxylases.
    Anal Biochem. 1972 Jan;45(1):242-52 PMID: 4621556
  36. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.6.475-485.1981
Published
1981-06-00
Pages
475-85
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369691
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.1.6.475-485.1981 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2