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PMID: 17065552 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

NPFXD-mediated endocytosis is required for polarity and function of a yeast cell wall stress sensor.

Molecular biology of the cell ·Vol. 18 ·No. 1 ·2007-01-00 ·Pages 57-65

Piao HL, Machado IM, Payne GS

Abstract

The actin-associated protein Sla1p, through its SHD1 domain, acts as an adaptor for the NPFX(1,2)D endocytic targeting signal in yeast. Here we report that Wsc1p, a cell wall stress sensor, depends on this signal-adaptor pair for endocytosis. Mutation of NPFDD in Wsc1p or expression of Sla1p lacking SHD1 blocked Wsc1p internalization. By live cell imaging, endocytically defective Wsc1p was not concentrated at sites of endocytosis. Polarized distribution of Wsc1p to regions of cell growth was lost in the absence of endocytosis. Mutations in genes necessary for endosome to Golgi traffic caused redistribution of Wsc1p from the cell surface to internal compartments, indicative of recycling. Inhibition of Wsc1p endocytosis caused defects in polarized deposition of the cell wall and increased sensitivity to perturbation of cell wall synthesis. Our results reveal that the NPFX(1,2)D-Sla1p system is responsible for directing Wsc1p into an endocytosis and recycling pathway necessary to maintain yeast cell wall polarity. The dynamic localization of Wsc1p, a sensor of the extracellular wall in yeast, resembles polarized distribution of certain extracellular matrix-sensing integrins through endocytic recycling.

MeSH Terms
Amino Acid Motifs Carrier Proteins/metabolism Cell Membrane/metabolism Cell Polarity Cell Wall/metabolism,ultrastructure Cytoskeletal Proteins Endocytosis Membrane Proteins/chemistry,metabolism Peptide Fragments/chemistry,metabolism Protein Binding Protein Structure, Tertiary Protein Transport Saccharomyces cerevisiae/cytology,ultrastructure Saccharomyces cerevisiae Proteins/chemistry,metabolism Signal Transduction
Chemicals
Carrier Proteins Cytoskeletal Proteins Membrane Proteins Peptide Fragments SLA1 protein, S cerevisiae SLG1 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Piao Hai Lan
Department of Biological Chemistry, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA 90095, USA.
Machado Iara M P
Payne Gregory S
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-08-0721
Published
2007-01-00
Epub
2006-00-25
Pages
57-65
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1751320
Subset
IM
Grants
NIGMS NIH HHS · R01 GM039040 · United States
NIGMS NIH HHS · GM39040 · United States
External Links
PubMed source DOI: 10.1091/mbc.e06-08-0721 PMC Full Text View journal details
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