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PMID: 1705037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multimerization of AAGTGA and GAAAGT generates sequences that mediate virus inducibility by mimicking an interferon promoter element.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 4 ·1991-02-15 ·Pages 1369-73

Näf D, Hardin SE, Weissmann C

Abstract

Multimeric AAGTGA and GAAAGT, when inserted before a minimal promoter, mediate virus-inducible transcription. We have determined that the active sequence within these multimers is TGAAAGTGAAAGT, which is structurally similar to GAGAAGTGAAAGT, a positive response element delineated in the beta-interferon gene promoter. Both sequences behave like protoenhancers and are similar as regards induction by virus or interferon regulatory factor 1 when supported by a simian virus 40 enhancer.

MeSH Terms
Animals Base Sequence Globins/genetics Interferon Inducers Interferons/genetics L Cells/physiology Mice Molecular Sequence Data Mutagenesis, Insertional Newcastle disease virus/genetics Oligodeoxyribonucleotides/chemical synthesis,pharmacology Oligonucleotide Probes Promoter Regions, Genetic TATA Box Transcription, Genetic/drug effects Transfection
Chemicals
Interferon Inducers Oligodeoxyribonucleotides Oligonucleotide Probes Globins Interferons
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Näf D
Institut für Molekularbiologie I. Universität Zürich, Switzerland.
Hardin S E
Weissmann C
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.4.1369
Published
1991-02-15
Pages
1369-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51019
Subset
IM
Databases
GENBANK
J00218, M57699, M57700, M57701, M57702, S74653, S74655, S74658, S74661, S74663
External Links
PubMed source DOI: 10.1073/pnas.88.4.1369 PMC Full Text View journal details
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