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PMID: 2850164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for a nuclear factor(s), IRF-1, mediating induction and silencing properties to human IFN-beta gene regulatory elements.

The EMBO journal ·Vol. 7 ·No. 11 ·1988-11-00 ·Pages 3397-405

Fujita T, Sakakibara J, Sudo Y, Miyamoto M, Kimura Y, Taniguchi T

Abstract

Transcription of the human interferon-beta (IFN-beta) gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. In this study, we describe a nuclear factor, termed interferon regulatory factor-1 (IRF-1), that is involved in the transcription of IFN-beta and possibly other genes. We demonstrate that IRF-1 functions in virus-induced transcription by interacting with previously identified, IFN-beta regulatory DNA elements. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex(es) with the regulatory elements. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells.

MeSH Terms
Animals Base Sequence Cell Line DNA/genetics,metabolism DNA Probes Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Humans Interferon Type I/genetics Molecular Sequence Data Mutation Nuclear Proteins/genetics,metabolism Plasmids Restriction Mapping Simian virus 40/genetics Transcription Factors/genetics,metabolism Transcription, Genetic Transfection
Chemicals
DNA Probes Interferon Type I Nuclear Proteins Transcription Factors DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fujita T
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Sakakibara J
Sudo Y
Miyamoto M
Kimura Y
Taniguchi T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-11-00
Pages
3397-405
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454838
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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