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PMID: 2370859 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synergism between distinct enhanson domains in viral induction of the human beta interferon gene.

Molecular and cellular biology ·Vol. 10 ·No. 8 ·1990-08-00 ·Pages 3987-93

Leblanc JF, Cohen L, Rodrigues M, Hiscott J

Abstract

This study demonstrates distinct virus-inducible enhanson properties for three regions of the human beta interferon (IFN-beta) promoter; maximum virus inducibility required syngerism among all three enhansons. Expression of the IRF-1 transcription factor differentially increased the expression of plasmids containing (AAGTGA)4 or PRDIII (-94 to -78) motifs but was inefficient in the induction of the intact IFN-beta promoter. The human T-cell lymphotropic virus type I Tax protein was a strong positive activator of PRDII (-64 to -55)-containing plasmids but was also unable to stimulate the IFN-beta promoter. Induction of the intact IFN-beta promoter linked to a reporter plasmid was achieved in lymphoid and epithelioid cellular backgrounds by a triple transfection with IRF-1 and Tax expression plasmids or a combination of IRF-1 and phorbol ester, indicating that at least two trans-activating events and the association of two proteins on the promoter template are required for IFN-beta activation.

MeSH Terms
Acetylation Base Sequence Cell Line DNA-Binding Proteins/genetics,metabolism Enhancer Elements, Genetic Gene Expression HIV/genetics Human T-lymphotropic virus 1/genetics Humans Interferon Inducers Interferon Regulatory Factor-1 Interferon Type I/genetics Molecular Sequence Data Oligonucleotide Probes Phosphoproteins/genetics,metabolism Plasmids Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Trans-Activators/genetics,metabolism Transcription Factors/genetics Transfection
Chemicals
DNA-Binding Proteins IRF1 protein, human Interferon Inducers Interferon Regulatory Factor-1 Interferon Type I Oligonucleotide Probes Phosphoproteins Trans-Activators Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leblanc J F
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.
Cohen L
Rodrigues M
Hiscott J
References (49)
49 references, click to expand
  1. Identification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4700-4 PMID: 3133660
  2. HTLV-1 transactivator induces interleukin-2 receptor expression through an NF-kappa B-like factor.
    Nature. 1988 Jun 23;333(6175):776-8 PMID: 2838755
  3. Regulated expression of a gene encoding a nuclear factor, IRF-1, that specifically binds to IFN-beta gene regulatory elements.
    Cell. 1988 Sep 9;54(6):903-13 PMID: 3409321
  4. Cooperativity and hierarchical levels of functional organization in the SV40 enhancer.
    Cell. 1988 Sep 23;54(7):943-53 PMID: 2843294
  5. HTLV-I tax induces cellular proteins that activate the kappa B element in the IL-2 receptor alpha gene.
    Science. 1988 Sep 23;241(4873):1652-5 PMID: 2843985
  6. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  7. Evidence for a nuclear factor(s), IRF-1, mediating induction and silencing properties to human IFN-beta gene regulatory elements.
    EMBO J. 1988 Nov;7(11):3397-405 PMID: 2850164
  8. Multiple protein-DNA interactions within the human interferon-beta regulatory element.
    J Biol Chem. 1989 Jan 15;264(2):1139-45 PMID: 2910848
  9. Induction of endogenous IFN-alpha and IFN-beta genes by a regulatory transcription factor, IRF-1.
    Nature. 1989 Jan 19;337(6204):270-2 PMID: 2911367
  10. Sequence requirement for specific interaction of an enhancer binding protein (EBP1) with DNA.
    Nucleic Acids Res. 1989 Jan 25;17(2):499-516 PMID: 2536920
  11. The involvement of NF-kappa B in beta-interferon gene regulation reveals its role as widely inducible mediator of signal transduction.
    Cell. 1989 Apr 21;57(2):287-94 PMID: 2495183
  12. Two different virus-inducible elements are required for human beta-interferon gene regulation.
    EMBO J. 1989 Jan;8(1):101-10 PMID: 2540952
  13. Kappa B-specific DNA binding proteins: role in the regulation of human interleukin-2 gene expression.
    Science. 1989 Apr 28;244(4903):457-60 PMID: 2497518
  14. Functionally distinct NF-kappa B binding sites in the immunoglobulin kappa and IL-2 receptor alpha chain genes.
    Science. 1989 Apr 28;244(4903):466-9 PMID: 2497520
  15. Induction of human interferon gene expression is associated with a nuclear factor that interacts with the NF-kappa B site of the human immunodeficiency virus enhancer.
    J Virol. 1989 Jun;63(6):2557-66 PMID: 2542571
  16. Involvement of a cis-element that binds an H2TF-1/NF kappa B like factor(s) in the virus-induced interferon-beta gene expression.
    Nucleic Acids Res. 1989 May 11;17(9):3335-46 PMID: 2566973
  17. Double-stranded RNA activates binding of NF-kappa B to an inducible element in the human beta-interferon promoter.
    EMBO J. 1989 Apr;8(4):1129-38 PMID: 2663471
  18. A gamma-interferon-induced factor that binds the interferon response sequence of the MHC class I gene, H-2Kb.
    EMBO J. 1989 Apr;8(4):1139-44 PMID: 2501084
  19. Modularity in promoters and enhancers.
    Cell. 1989 Jul 14;58(1):1-4 PMID: 2665940
  20. In vitro activation and nuclear translocation of NF-kappa B catalyzed by cyclic AMP-dependent protein kinase and protein kinase C.
    Mol Cell Biol. 1989 Jun;9(6):2424-30 PMID: 2548081
  21. Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes.
    Cell. 1989 Aug 25;58(4):729-39 PMID: 2475256
  22. Transient induction of IL-2 receptor in cultured T cell lines by HTLV-1 LTR-linked tax-1 gene.
    EMBO J. 1989 Jul;8(7):1953-8 PMID: 2792077
  23. The SV40 TC-II(kappa B) and the related H-2Kb enhansons exhibit different cell type specific and inducible proto-enhancer activities, but the SV40 core sequence and the AP-2 binding site have no enhanson properties.
    EMBO J. 1989 Dec 20;8(13):4205-14 PMID: 2556264
  24. The SV40 TC-II(kappa B) enhanson binds ubiquitous and cell type specifically inducible nuclear proteins from lymphoid and non-lymphoid cell lines.
    EMBO J. 1989 Dec 20;8(13):4215-27 PMID: 2556265
  25. Induction of the transcription factor IRF-1 and interferon-beta mRNAs by cytokines and activators of second-messenger pathways.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9936-40 PMID: 2557635
  26. A 65-kappaD subunit of active NF-kappaB is required for inhibition of NF-kappaB by I kappaB.
    Genes Dev. 1989 Nov;3(11):1689-98 PMID: 2691328
  27. A DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence.
    Genes Dev. 1990 Jan;4(1):29-42 PMID: 2106471
  28. Different pathways mediate virus inducibility of the human IFN-alpha 1 and IFN-beta genes.
    Cell. 1990 Mar 9;60(5):767-79 PMID: 2107026
  29. Detection of factors that interact with the human beta-interferon regulatory region in vivo by DNAase I footprinting.
    Cell. 1986 May 23;45(4):611-8 PMID: 3708689
  30. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  31. An inducible transcription factor activates expression of human immunodeficiency virus in T cells.
    Nature. 1987 Apr 16-22;326(6114):711-3 PMID: 3031512
  32. Interferon-beta gene regulation: tandemly repeated sequences of a synthetic 6 bp oligomer function as a virus-inducible enhancer.
    Cell. 1987 May 8;49(3):357-67 PMID: 3032451
  33. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  34. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  35. Differential expression of human interferon genes.
    Nucleic Acids Res. 1984 May 11;12(9):3727-46 PMID: 6427755
  36. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene.
    Cell. 1985 Jun;41(2):489-96 PMID: 2985279
  37. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements.
    Cell. 1985 Jul;41(3):885-97 PMID: 3924411
  38. Dideoxy sequencing method using denatured plasmid templates.
    Anal Biochem. 1986 Feb 1;152(2):232-8 PMID: 3516005
  39. The human beta-interferon gene enhancer is under negative control.
    Cell. 1986 May 23;45(4):601-10 PMID: 3708688
  40. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  41. Cooperative interaction of multiple DNA elements in the human interferon-beta promoter.
    Eur J Biochem. 1987 Jul 1;166(1):103-9 PMID: 3036515
  42. Transient expression of the beta interferon promoter in human cells.
    Mol Cell Biol. 1987 Oct;7(10):3830-5 PMID: 2824999
  43. Activation of the HIV-1 LTR by T cell mitogens and the trans-activator protein of HTLV-I.
    Science. 1987 Dec 11;238(4833):1575-8 PMID: 2825351
  44. Two transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter.
    Proc Natl Acad Sci U S A. 1988 Feb;85(3):723-7 PMID: 3422454
  45. Overlapping positive and negative regulatory domains of the human beta-interferon gene.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1447-51 PMID: 3422743
  46. The SV40 enhancer contains two distinct levels of organization.
    Nature. 1988 May 5;333(6168):40-5 PMID: 2834649
  47. Identification of an inducible factor that binds to a positive regulatory element of the human beta-interferon gene.
    Proc Natl Acad Sci U S A. 1988 May;85(10):3309-13 PMID: 3368441
  48. The same inducible nuclear proteins regulates mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIV.
    Cell. 1988 Jun 3;53(5):827-36 PMID: 2836068
  49. Cellular transcription factors and regulation of IL-2 receptor gene expression by HTLV-I tax gene product.
    Science. 1988 Jul 1;241(4861):89-92 PMID: 2838905
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.8.3987-3993.1990
Published
1990-08-00
Pages
3987-93
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360909
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.8.3987-3993.1990 PMC Full Text View journal details
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