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PMID: 1700693 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparative studies on tat mutants of three primate lentiviruses.

Archives of virology ·Vol. 114 ·No. 3-4 ·1990-00-00 ·Pages 243-50

Shibata R, Sakai H, Ogawa K, Ishimoto A, Adachi A

Abstract

A frame-shift tat gene mutant of human immunodeficiency virus type 1 (HIV-1), which showed no detectable trans-activation potential, was constructed in vitro. Upon transfection, this clone directed the synthesis of virus mRNAs, gag proteins and virion-associated reverse transcriptase (RT) at a low level as was observed with the tat mutants of HIV-2 and simian immunodeficiency virus isolated from African green monkey (SIVAGM). Using these mutant viruses, trans-activation efficiency of viral gene expression by tat was compared among HIV-1, HIV-2, and SIVAGM. SIVAGM seemed to be less dependent on tat for RT production than HIV-1 and HIV-2.

MeSH Terms
Base Sequence DNA, Viral Frameshift Mutation Gene Expression Regulation, Viral Genes, tat HIV-1/genetics HIV-2/genetics Humans Molecular Sequence Data Proviruses/genetics RNA-Directed DNA Polymerase/biosynthesis,genetics Simian Immunodeficiency Virus/genetics Trans-Activators Transfection Tumor Cells, Cultured
Chemicals
DNA, Viral Trans-Activators RNA-Directed DNA Polymerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shibata R
Department of Viral Oncology, Kyoto University, Japan.
Sakai H
Ogawa K
Ishimoto A
Adachi A
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34 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01310753
Published
1990-00-00
Pages
243-50
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01310753 View journal details
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