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PMID: 1697035 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.

Molecular and cellular biology ·Vol. 10 ·No. 9 ·1990-09-00 ·Pages 4987-9

Singer-Sam J, Grant M, LeBon JM, Okuyama K, Chapman V, Monk M, Riggs AD

Abstract

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

MeSH Terms
Animals Base Sequence DNA/genetics Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific Dinucleoside Phosphates Embryo, Mammalian/physiology Female Male Methylation Mice Molecular Sequence Data Oligonucleotide Probes Phosphoglycerate Kinase/genetics Polymerase Chain Reaction Restriction Mapping X Chromosome
Chemicals
Dinucleoside Phosphates Oligonucleotide Probes cytidylyl-3'-5'-guanosine DNA Phosphoglycerate Kinase Deoxyribonuclease HpaII Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Singer-Sam J
Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010.
Grant M
LeBon J M
Okuyama K
Chapman V
Monk M
Riggs A D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.9.4987-4989.1990
Published
1990-09-00
Pages
4987-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361130
Subset
IM
Grants
NIA NIH HHS · AG08196-01 · United States
NIGMS NIH HHS · GM24125 · United States
NIGMS NIH HHS · GM33160 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.9.4987-4989.1990 PMC Full Text View journal details
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