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PMID: 1690874 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis.

Nucleic acids research ·Vol. 18 ·No. 5 ·1990-03-11 ·Pages 1255-9

Singer-Sam J, Robinson MO, Bellvé AR, Simon MI, Riggs AD

Abstract

A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was used quantitatively to measure accumulated levels of RNA transcripts in total mouse RNAs derived from male germ cells at various spermatogenic stages. RNA levels for two X-linked enzymes, phosphoglycerate kinase (PGK-1) and hypoxanthine phosphoribosyl transferase (HPRT), both decrease during spermatogenesis, although the transcript levels decrease much more rapidly for PGK-1. RNA for the Y-linked ZFY (zinc finger protein) is elevated in all spermatogenic cell fractions tested, being particularly high in leptotene/zygotene spermatocytes and round spermatids. RNA for adenine phosphoribosyltransferase (APRT) increases 5-fold to a peak during late pachynema. RNA for PGK-2, undetectable in spermatogonial cells, increases at least 50-fold by the round spermatid stage. DNA (cytosine-5-)-methyltransferase (MTase) transcript levels are over an order of magnitude higher throughout spermatogenesis than in non-dividing liver cells.

MeSH Terms
Adenine Phosphoribosyltransferase/genetics Animals Base Sequence DNA (Cytosine-5-)-Methyltransferases/genetics DNA-Binding Proteins/genetics Genes Hypoxanthine Phosphoribosyltransferase/genetics Male Metalloproteins/genetics Mice Molecular Sequence Data Phosphoglycerate Kinase/genetics Polymerase Chain Reaction RNA/analysis,genetics Spermatogenesis/genetics Transcription, Genetic Zinc
Chemicals
DNA-Binding Proteins Metalloproteins RNA DNA (Cytosine-5-)-Methyltransferases Adenine Phosphoribosyltransferase Hypoxanthine Phosphoribosyltransferase Phosphoglycerate Kinase Zinc
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Singer-Sam J
Division of Biology, Beckman Research Institute, City of Hope, Duarte, CA 91010.
Robinson M O
Bellvé A R
Simon M I
Riggs A D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.5.1255
Published
1990-03-11
Pages
1255-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330442
Subset
IM
Grants
NIA NIH HHS · 1 R01 AG08196-01 · United States
NICHD NIH HHS · NICHHD 05077 · United States
External Links
PubMed source DOI: 10.1093/nar/18.5.1255 PMC Full Text View journal details
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