In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS.

Plant physiology ·Vol. 69 ·No. 1 ·1982-01-00 ·Pages 150-4

Boston RS, Miller TJ, Mertz JE, Burgess RR

Abstract

Wheat (Triticum aestivum) RNA was used to program synthesis of the alpha-amylase protein by Xenopus laevis oocytes. A 41,500-dalton protein was made which was identified as alpha-amylase by immunoprecipitation with rabbit anti-alpha-amylase antiserum raised against the purified wheat protein and by its co-migration with authentic alpha-amylase on sodium dodecyl sulfate polyacrylamide gels. Synthesis of alpha-amylase was dependent upon injection of RNA extracted from gibberellic acid-induced aleurone layers from wheat. The amount of alpha-amylase produced was proportional to the amount of RNA injected and reached a plateau within 4 hours after injection. When the same RNA was translated in a wheat germ cell-free translation system, a 43,000-dalton protein was produced. Addition of dog pancreas microsomal membranes to the wheat germ translation system resulted in processing of the alpha-amylase protein to a form which co-migrated with authentic alpha-amylase purified from malted wheat and with the protein synthesized in oocytes.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Boston R S

McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706.

Miller T J

Mertz J E

Burgess R R

References (35)

35 references, click to expand

The sequestration, processing and retention of honey-bee promelittin made in amphibian oocytes.
Eur J Biochem. 1981 Jan;113(2):273-81 PMID: 7193578
Control of protein synthesis in barley aleurone layers by the plant hormones gibberellic acid and abscisic acid.
Cell. 1980 Jun;20(2):479-85 PMID: 6156008
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
Delimitation of a promoter for RNA polymerase III by means of a functional test.
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2590-4 PMID: 288049
The influence of topology and glycosylation on the fate of heterologous secretory proteins made in Xenopus oocytes.
Eur J Biochem. 1981 Jan;113(2):339-48 PMID: 6162638
Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3665-9 PMID: 4345506
Synthesis of carp proinsulin in Xenopus oocytes.
Eur J Biochem. 1978 Jun 15;87(2):229-33 PMID: 668692
A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520-6 PMID: 5237885
Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes.
J Biol Chem. 1978 Jun 10;253(11):3753-6 PMID: 649601
Subcellular compartmentalization of maize storage proteins in Xenopus oocytes injected with zein messenger RNAs.
J Cell Biol. 1981 May;89(2):292-9 PMID: 7251653
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
Precursor Forms of Pea Vicilin Subunits: MODIFICATION BY MICROSOMAL MEMBRANES DURING CELL-FREE TRANSLATION.
Plant Physiol. 1981 Feb;67(2):205-11 PMID: 16661647
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
J Embryol Exp Morphol. 1976 Dec;36(3):523-40 PMID: 1010977
Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue.
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3290-3 PMID: 4528576
Synthesis and processing of maize storage proteins in Xenopus laevis oocytes.
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6448-52 PMID: 16592743
The isolation of ribonucleic acid from plant, bacterial or animal cells.
Biochim Biophys Acta. 1966 Oct 24;129(1):74-84 PMID: 5339108
Processing of placental peptide hormones synthesized in lysates containing membranes derived from tunicamycin-treated ascites tumor cells.
J Biol Chem. 1978 Oct 25;253(20):7117-9 PMID: 568137
Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3278-82 PMID: 198778
Kinetics of synthesis and processing of precursor polypeptides of murine leukemia virus in frog oocytes following microinjection of viral RNA.
Eur J Biochem. 1980 Aug;109(2):395-403 PMID: 7408891
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Translation of plant-specific messenger RNAs in living animal cells.
FEBS Lett. 1977 Sep 1;81(1):10-2 PMID: 332519
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
J Mol Biol. 1979 Dec 15;135(3):709-32 PMID: 537089
Coupled transcription-translation of DNA injected into Xenopus oocytes.
Cell. 1977 Sep;12(1):175-82 PMID: 198138
Partial amino acid sequence of human placental lactogen precursor and its mature hormone form produced by membrane-associated enzyme activity.
Biochem Biophys Res Commun. 1977 Jan 10;74(1):106-12 PMID: 836272
An efficient mRNA-dependent translation system from reticulocyte lysates.
Eur J Biochem. 1976 Aug 1;67(1):247-56 PMID: 823012
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
Nature. 1980 Mar 27;284(5754):316-20 PMID: 6987533
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 PMID: 4525168
Thyroglobulin messenger RNA: translation of a 33-S mRNA into a peptide immunologically related to thyroglobulin.
Eur J Biochem. 1975 Jun 16;55(1):15-22 PMID: 1236796
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
Eur J Biochem. 1980 Oct;111(1):225-35 PMID: 7439186
Processing in vitro of placental peptide hormones by smooth microsomes.
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6152-6 PMID: 293711
Partial purification and characterization of the mRNA for alpha-amylase from barley aleurone layers.
Plant Physiol. 1980 May;65(5):834-7 PMID: 16661291
Synthesis of a possible precursor of alpha-amylase in wheat aleurone cells.
Plant Physiol. 1979 Jan;63(1):195-200 PMID: 16660677
Ribonuclease inhibitor from human placenta: rapid purification and assay.
J Biol Chem. 1979 Dec 25;254(24):12484-7 PMID: 500726
Cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes.
Proc Natl Acad Sci U S A. 1977 May;74(5):2059-63 PMID: 325565
Synthesis of Dictyostelium discoideum secretory proteins in Xenopus laevis oocytes.
FEBS Lett. 1979 Aug 15;104(2):275-8 PMID: 477987

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.69.1.150

Published

1982-01-00

Pages

150-4

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC426165

External Links

PubMed source DOI: 10.1104/pp.69.1.150 PMC Full Text View journal details

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In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS.

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