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PMID: 16660677 Published · ppublish English Journal Article

Synthesis of a possible precursor of alpha-amylase in wheat aleurone cells.

Plant physiology ·Vol. 63 ·No. 1 ·1979-01-00 ·Pages 195-200

Okita TW, Decaleya R, Rappaport L

Abstract

alpha-Amylase from wheat aleurone (Triticum aestivum) was synthesized in a S-150 wheat germ readout system using polysomes, and a messenger RNA-dependent reticulocyte lysate system using polyadenylic acid [poly(A)]-enriched RNA. The product was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, precipitation by specific lambda-globulin for alpha-amylase, and proteolysis. Two immunoprecipitated products were synthesized from the readout system, the predominant species migrating coincidentally with authentic alpha-amylase on sodium dodecyl sulfate-polyacrylamide gels. A putative precursor, 1,500 daltons larger, was evident but was less abundant. The relationship between the two polypeptides was established by proteolytic analysis using Staphylococcus aureus V8 protease. At least nine fragments were generated and were identical in both species. The poly(A)-enriched RNA synthesized only the putative precursor in the reticulocyte lysate system. Attempts to process the precursor to the mature size of alpha-amylase failed. These findings are discussed in connection with the signal hypothesis (proposed for the transport of proteins across membranes) and the mode of secretion of alpha-amylase in aleurone cells.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Okita T W
Plant Growth Laboratory and Department of Vegetable Crops, University of California, Davis, California 95616.
Decaleya R
Rappaport L
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.63.1.195
Published
1979-01-00
Pages
195-200
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC542793
External Links
PubMed source DOI: 10.1104/pp.63.1.195 PMC Full Text View journal details
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