Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 12 ·1972-12-00 ·Pages 3665-9

Laskey RA, Gurdon JB, Crawford LV

Abstract

RNA from encephalomyocarditis virus was injected into oocytes of Xenopus laevis. After incubation of the oocytes in [(35)S]methionine, polyacrylamide gel electrophoresis showed that a new series of polypeptides had been synthesized. They were identical in size to the polypeptides that appeared in ascites cells after infection with this virus. Electrophoretic and chromatographic analysis of the methionine-containing tryptic peptides from three of the induced polypeptides confirmed that they were virus-specific. All of the bands that appeared in ascites cells after infection also appeared in oocytes after injection of RNA from the virus. We conclude that Xenopus oocytes translate a mammalian viral mRNA faithfully and extensively, and perform normal post-translational modifications.

MeSH Terms

Animals Cells, Cultured Chromatography, Thin Layer Electrophoresis Electrophoresis, Polyacrylamide Gel Encephalomyocarditis virus/metabolism Female Methionine/metabolism Molecular Weight Ovum/metabolism Peptides/analysis Protein Biosynthesis RNA, Viral/metabolism Sulfur Isotopes Viral Proteins/analysis Xenopus

Chemicals

Peptides RNA, Viral Sulfur Isotopes Viral Proteins Methionine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Laskey R A

Gurdon J B

Crawford L V

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.12.3665

Published

1972-12-00

Pages

3665-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389844

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.12.3665 PMC Full Text View journal details

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Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis.

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