Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3290-3

Jacobsen JV, Zwar JA

Abstract

Incubation of isolated barley aleurone layers with gibberellic acid for 16 hr caused a 50% increase in the synthesis of RNA that contains poly(A) sequences [poly(A)-RNA], but had no measurable effect on the syntheses of the major RNA species. The syntheses of both the poly(A) and the heteropolymeric fractions of the poly(A)-RNA were increased. The poly(A) sequences were separated into two classes by size, one containing an average of 250 nucleotides and the other about 70 nucleotides. The two classes occurred in a molar ratio of about 1:1. Gibberellic acid increased the syntheses of both sequences to the same extent. We interpret these results to mean that gibberellic acid increases specifically the synthesis of mRNA in this tissue.

MeSH Terms

Adenine Nucleotides/isolation & purification Base Sequence Carbon Radioisotopes Electrophoresis, Polyacrylamide Gel Gibberellins/pharmacology Hordeum/metabolism Phosphorus Radioisotopes Plants/metabolism Polynucleotides/isolation & purification RNA/analysis,biosynthesis RNA, Messenger/biosynthesis Stimulation, Chemical Tritium

Chemicals

Adenine Nucleotides Carbon Radioisotopes Gibberellins Phosphorus Radioisotopes Polynucleotides RNA, Messenger Tritium RNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jacobsen J V

Zwar J A

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.8.3290

Published

1974-08-00

Pages

3290-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388671

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.8.3290 PMC Full Text View journal details

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Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue.

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