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PMID: 1661372 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restored DNA-binding of the cAMP-CRP activator complex reestablishes negative regulation by the CytR repressor in the deoP2 promoter in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 231 ·No. 1 ·1991-12-00 ·Pages 76-80

Søgaard-Andersen L, Valentin-Hansen P

Abstract

We have investigated in vivo the coupling between CytR regulation of the deoP2 promoter in Escherichia coli and the DNA-binding specificity of the cAMP-CRP (cAMP receptor protein) complex in order to obtain a more detailed picture of the role played by cAMP-CRP in CytR regulation. By introducing CRP proteins that exhibit an altered DNA binding specificity into a strain containing a mutant deoP2 promoter in which cAMP-CRP activation was decreased and CytR regulation completely abolished, we show that CytR regulation of this promoter can be reestablished by restored the DNA binding of the cAMP-CRP complex. Hence, CytR regulation of deoP2 can be modulated by simply varying DNA binding of cAMP-CRP. These data confirm the crucial role played by the cAMP-CRP activator complex in CytR regulation of the deoP2 promoter.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence Carrier Proteins/metabolism Cyclic AMP/metabolism Cyclic AMP Receptor Protein DNA, Bacterial/metabolism DNA-Binding Proteins/metabolism Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial/genetics Molecular Sequence Data Plasmids/genetics Promoter Regions, Genetic/physiology Receptors, Cyclic AMP/metabolism Recombinant Fusion Proteins/biosynthesis Repressor Proteins/metabolism,physiology Single-Strand Specific DNA and RNA Endonucleases/metabolism beta-Galactosidase/genetics,metabolism
Chemicals
Bacterial Proteins Carrier Proteins Cyclic AMP Receptor Protein CytR protein, E coli DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Receptors, Cyclic AMP Recombinant Fusion Proteins Repressor Proteins Cyclic AMP Single-Strand Specific DNA and RNA Endonucleases beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Søgaard-Andersen L
Department of Molecular Biology, Odense University, Denmark.
Valentin-Hansen P
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27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00293824
Published
1991-12-00
Pages
76-80
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00293824
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