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PMID: 16611747 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Members of the NIMA-related kinase family promote disassembly of cilia by multiple mechanisms.

Molecular biology of the cell ·Vol. 17 ·No. 6 ·2006-06-00 ·Pages 2799-810

Wloga D, Camba A, Rogowski K, Manning G, Jerka-Dziadosz M, Gaertig J

Abstract

The genome of Tetrahymena thermophila contains 39 loci encoding NIMA-related kinases (NRKs), an extraordinarily large number for a unicellular organism. Evolutionary analyses grouped these sequences into several subfamilies, some of which have orthologues in animals, whereas others are protist specific. When overproduced, NRKs of three subfamilies caused rapid shortening of cilia. Ultrastructural studies revealed that each NRK triggered ciliary resorption by a distinct mechanism that involved preferential depolymerization of a subset of axonemal microtubules, at either the distal or proximal end. Overexpression of a kinase-inactive variant caused lengthening of cilia, indicating that constitutive NRK-mediated resorption regulates the length of cilia. Each NRK preferentially resorbed a distinct subset of cilia, depending on the location along the anteroposterior axis. We also show that normal Tetrahymena cells maintain unequal length cilia. We propose that ciliates used a large number of NRK paralogues to differentially regulate the length of specific subsets of cilia in the same cell.

MeSH Terms
Animals Cell Cycle Proteins/classification,genetics,metabolism Cilia/physiology Genes, Protozoan Genes, Reporter Genome Green Fluorescent Proteins/genetics NIMA-Related Kinase 1 Phylogeny Protein Serine-Threonine Kinases/classification,genetics,metabolism Protozoan Proteins/chemistry,metabolism Tetrahymena thermophila/physiology,ultrastructure
Chemicals
Cell Cycle Proteins Protozoan Proteins Green Fluorescent Proteins NIMA-Related Kinase 1 Protein Serine-Threonine Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wloga Dorota
Department of Cellular Biology, University of Georgia, Athens, GA 30602-2607, USA.
Camba Amy
Rogowski Krzysztof
Manning Gerard
Jerka-Dziadosz Maria
Gaertig Jacek
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e05-05-0450
Published
2006-06-00
Epub
2006-00-12
Pages
2799-810
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1474788
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e05-05-0450 PMC Full Text View journal details
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