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PMID: 15371535 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells.

Molecular biology of the cell ·Vol. 15 ·No. 11 ·2004-11-00 ·Pages 5172-86

Mahjoub MR, Qasim Rasi M, Quarmby LM

Abstract

Polycystic kidney disease and related syndromes involve dysregulation of cell proliferation in conjunction with ciliary defects. The relationship between cilia and cell cycle is enigmatic, but it may involve regulation by the NIMA-family of kinases (Neks). We previously showed that the Nek Fa2p is important for ciliary function and cell cycle in Chlamydomonas. We now show that Fa2p localizes to an important regulatory site at the proximal end of cilia in both Chlamydomonas and a mouse kidney cell line. Fa2p also is associated with the proximal end of centrioles. Its localization is dynamic during the cell cycle, following a similar pattern in both cell types. The cell cycle function of Fa2p is kinase independent, whereas its ciliary function is kinase dependent. Mice with mutations in Nek1 or Nek8 have cystic kidneys; therefore, our discovery that a member of this phylogenetic group of Nek proteins is localized to the same sites in Chlamydomonas and kidney epithelial cells suggests that Neks play conserved roles in the coordination of cilia and cell cycle progression.

MeSH Terms
Animals Cell Cycle Cell Cycle Proteins/chemistry,physiology Cell Line Centrioles/metabolism,ultrastructure Chlamydomonas/metabolism Cilia/metabolism DNA, Complementary/metabolism Epitopes/chemistry Fluorescent Antibody Technique, Indirect Green Fluorescent Proteins/metabolism Immunoblotting Kidney/metabolism,pathology Mice Mitosis Mutation NIMA-Related Kinase 1 NIMA-Related Kinases Protein Kinases/metabolism Protein Serine-Threonine Kinases/biosynthesis,chemistry,metabolism,physiology Protein Structure, Tertiary Protein-Tyrosine Kinases/metabolism Signal Transduction Subcellular Fractions/metabolism Time Factors
Chemicals
Cell Cycle Proteins DNA, Complementary Epitopes Green Fluorescent Proteins Protein Kinases Protein-Tyrosine Kinases NIMA-Related Kinase 1 NIMA-Related Kinases Nek1 protein, mouse Nek8 protein, mouse Protein Serine-Threonine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mahjoub Moe R
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada.
Qasim Rasi M
Quarmby Lynne M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-07-0571
Published
2004-11-00
Epub
2004-00-15
Pages
5172-86
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC524795
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e04-07-0571 PMC Full Text View journal details
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