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PMID: 8522608 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane.

The Journal of cell biology ·Vol. 131 ·No. 6 Pt 1 ·1995-12-00 ·Pages 1517-27

Kozminski KG, Beech PL, Rosenbaum JL

Abstract

The Chlamydomonas FLA10 gene was shown to encode a flagellar kinesin-like protein (Walther, Z., M. Vashishtha, and J.L. Hall. 1994. J. Cell Biol. 126:175-188). By using a temperature-sensitive allele of FLA10, we have determined that the FLA10 protein is necessary for both the bidirectional movement of polystyrene beads on the flagellar membrane and intraflagellar transport (IFT), the bidirectional movement of granule-like particles beneath the flagellar membrane (Kozminski, K.G., K.A. Johnson, P. Forscher, and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. (USA). 90:5519-5523). In addition, we have correlated the presence and position of the IFT particles visualized by light microscopy with that of the electron dense complexes (rafts) observed beneath the flagellar membrane by electron microscopy. A role for FLA10 in submembranous or flagellar surface motility is also strongly supported by the immunolocalization of FLA10 to the region between the axonemal outer doublet microtubules and the flagellar membrane.

MeSH Terms
Animals Antibody Specificity Base Sequence Cell Movement/physiology Chlamydomonas/cytology,physiology,ultrastructure Flagella/chemistry,physiology,ultrastructure Kinesins/immunology,physiology Membrane Proteins/physiology Microscopy, Immunoelectron Microscopy, Video Molecular Sequence Data Temperature
Chemicals
Membrane Proteins Kinesins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kozminski K G
Department of Biology, Yale University, New Haven, Connecticut 06511, USA.
Beech P L
Rosenbaum J L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.6.1517
Published
1995-12-00
Pages
1517-27
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120669
Subset
IM
Grants
NIGMS NIH HHS · GM14642 · United States
External Links
PubMed source DOI: 10.1083/jcb.131.6.1517 PMC Full Text View journal details
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