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PMID: 12925756 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hypoxia regulates assembly of cilia in suppressors of Tetrahymena lacking an intraflagellar transport subunit gene.

Molecular biology of the cell ·Vol. 14 ·No. 8 ·2003-08-00 ·Pages 3192-207

Brown JM, Fine NA, Pandiyan G, Thazhath R, Gaertig J

Abstract

We cloned a Tetrahymena thermophila gene, IFT52, encoding a homolog of the Chlamydomonas intraflagellar transport protein, IFT52. Disruption of IFT52 led to loss of cilia and incomplete cytokinesis, a phenotype indistinguishable from that of mutants lacking kinesin-II, a known ciliary assembly transporter. The cytokinesis failures seem to result from lack of cell movement rather than from direct involvement of ciliary assembly pathway components in cytokinesis. Spontaneous partial suppressors of the IFT52 null mutants occurred, which assembled cilia at high cell density and resorbed cilia at low cell density. The stimulating effect of high cell density on cilia formation is based on the creation of pericellular hypoxia. Thus, at least under certain conditions, ciliary assembly is affected by an extracellular signal and the Ift52p function may be integrated into signaling pathways that regulate ciliogenesis.

MeSH Terms
Algal Proteins/metabolism Amino Acid Sequence Animals Cell Count Cell Division Cell Hypoxia/physiology Cilia/metabolism,ultrastructure Kinesins/metabolism Microscopy, Electron Molecular Sequence Data Mutation Protozoan Proteins/metabolism Sequence Homology Tetrahymena thermophila/cytology,genetics
Chemicals
Algal Proteins Protozoan Proteins Kinesins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brown Jason M
Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA.
Fine Noah A
Pandiyan Gautham
Thazhath Rupal
Gaertig Jacek
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e03-03-0166
Published
2003-08-00
Epub
2003-00-03
Pages
3192-207
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC181560
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e03-03-0166 PMC Full Text View journal details
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