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PMID: 16601680 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter.

The EMBO journal ·Vol. 25 ·No. 8 ·2006-04-19 ·Pages 1690-9

Tréand C, du Chéné I, Brès V, Kiernan R, Benarous R, Benkirane M, Emiliani S

Abstract

Activation of the human immunodeficiency virus type-1 (HIV-1) promoter in infected cells requires the sequential recruitment of several cellular factors to facilitate the formation of a processive elongation complex. The nucleosomal reorganization of the HIV-1 long terminal repeat (LTR) observed upon Tat stimulation suggests that chromatin-remodeling complexes could play a role during this process. Here, we reported that Tat interacts directly with Brm, a DNA-dependent ATPase subunit of the SWI/SNF chromatin-remodeling complex, to activate the HIV-1 LTR. Inhibition of Brm via small interfering RNAs impaired Tat-mediated transactivation of an integrated HIV-1 promoter. Furthermore, Brm is recruited in vivo to the HIV-1 LTR in a Tat-dependent manner. Interestingly, we found that Tat/Brm interaction is regulated by Tat lysine 50 acetylation. These data show the requirement of Tat-mediated recruitment of SWI/SNF chromatin-remodeling complex to HIV-1 promoter in the activation of the LTR.

MeSH Terms
Acetylation Amino Acid Motifs Arginine/genetics Cell Line Gene Products, tat/genetics,physiology HIV-1/genetics Humans Lysine/metabolism Mutation Promoter Regions, Genetic Protein Binding Protein Subunits/genetics,physiology Terminal Repeat Sequences Transcription Factors/genetics,physiology tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Protein Subunits SMARCA2 protein, human Transcription Factors tat Gene Products, Human Immunodeficiency Virus Arginine Lysine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tréand Céline
Institut Cochin, Département de Génétique et Développement, Paris, France.
du Chéné Isaure
Brès Vanessa
Kiernan Rosemary
Benarous Richard
Benkirane Monsef
Emiliani Stéphane
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7601074
Published
2006-04-19
Epub
2006-00-06
Pages
1690-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1440843
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7601074 PMC Full Text View journal details
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