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PMID: 1656220 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 5016-31

O'Bryan JP, Frye RA, Cogswell PC, Neubauer A, Kitch B, Prokop C, Espinosa R, Le Beau MM, Earp HS, Liu ET

Abstract

Using a sensitive transfection-tumorigenicity assay, we have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the product of this gene, axl, is a receptor tyrosine kinase. Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic transformation with the concomitant appearance of a 140-kDa axl tyrosine-phosphorylated protein. Expression of axl cDNA in the baculovirus system results in the expression of the appropriate recombinant protein that is recognized by antiphosphotyrosine antibodies, confirming that the axl protein is a tyrosine kinase. The juxtaposition of fibronectin type III and immunoglobulinlike repeats in the extracellular domain, as well as distinct amino acid sequences in the kinase domain, indicate that the axl protein represents a novel subclass of receptor tyrosine kinases.

MeSH Terms
Amino Acid Sequence Animals Baculoviridae/metabolism Base Sequence Blotting, Northern Blotting, Southern Cell Transformation, Neoplastic/genetics Cloning, Molecular Fibronectins/genetics Gene Expression/physiology Humans Immunoglobulins/genetics Leukemia, Myeloid/genetics Mice Molecular Sequence Data Oncogene Proteins/genetics,metabolism Polymerase Chain Reaction Protein-Tyrosine Kinases/genetics,physiology Proto-Oncogene Proteins Receptor Protein-Tyrosine Kinases Receptors, Cell Surface/genetics,metabolism Sequence Alignment Tumor Cells, Cultured
Chemicals
Fibronectins Immunoglobulins Oncogene Proteins Proto-Oncogene Proteins Receptors, Cell Surface Protein-Tyrosine Kinases Receptor Protein-Tyrosine Kinases axl receptor tyrosine kinase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
O'Bryan J P
Curriculum in Genetics, Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27599.
Frye R A
Cogswell P C
Neubauer A
Kitch B
Prokop C
Espinosa R
Le Beau M M
Earp H S
Liu E T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.5016-5031.1991
Published
1991-10-00
Pages
5016-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361494
Subset
IM
Grants
NCI NIH HHS · R01-CA49240 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.10.5016-5031.1991 PMC Full Text View journal details
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