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PMID: 1655112 Published · ppublish English Journal Article

Strong negative and positive regulatory elements contribute to the high-level fruit-specific expression of the tomato 2A11 gene.

Plant molecular biology ·Vol. 17 ·No. 4 ·1991-10-00 ·Pages 615-30

Van Haaren MJ, Houck CM

Abstract

Fruit-specific expression of beta-glucuronidase (GUS) activity was produced in transgenic tomato plants when the GUS-coding region was flanked by 5' and 3' regions of the tomato 2A11 gene. Deletion studies on the 5' region revealed a number of strong regulatory elements involved in the proper expression of the 2A11 gene. A 4.0 kb and a 1.3 kb 5' region can confer high-level fruit-specific GUS expression, while a 1.8 kb 5' region produces no GUS activity in leaf or fruit tissue. Thus, a strong negative regulatory element is present in the region between 1324 bp and 1796 bp upstream of the 2A11 transcriptional start and a strong fruit-specific positive regulatory element is present more than 1.8 kb upstream of the transcriptional start site. The 1.8 kb promoter region can be activated by the upstream insertion of the CaMV 35S enhancer sequence, albeit not in a fruit-specific fashion. Substitution of the 3' region of the 2A11 gene with a different 3' region does not seem to affect GUS expression significantly, indicating a minor role, if any, for the 3' region in the fruit-specific expression of the 2A11 gene.

Related Genes
2A11
MeSH Terms
Agrobacterium tumefaciens/genetics Base Sequence Blotting, Northern DNA Mutational Analysis DNA Transposable Elements/genetics Drug Resistance/genetics Enhancer Elements, Genetic/genetics Escherichia coli/genetics Fruit/enzymology,genetics,growth & development Gene Expression/physiology Glucuronidase/genetics,metabolism Kanamycin/pharmacology Molecular Sequence Data Mosaic Viruses/genetics Plant Proteins/genetics Plants, Genetically Modified/enzymology,genetics,growth & development Promoter Regions, Genetic/genetics Regulatory Sequences, Nucleic Acid/genetics Restriction Mapping
Chemicals
DNA Transposable Elements Plant Proteins Kanamycin Glucuronidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Van Haaren M J
Calgene Inc., Davis, CA 95616.
Houck C M
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36 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00037048
Published
1991-10-00
Pages
615-30
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
M37631
External Links
PubMed source DOI: 10.1007/BF00037048 View journal details
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