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PMID: 2704733 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An erythroid-specific, developmental-stage-independent enhancer far upstream of the human "beta-like globin" genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 8 ·1989-04-00 ·Pages 2554-8

Tuan DY, Solomon WB, London IM, Lee DP

Abstract

We have identified an erythroid-specific enhancer element far upstream of the human "beta-like globin" genes, at 10.2-11.0 kilobases 5' of the embryonic epsilon-globin gene, and thus at 53-54 kilobases 5' of the adult beta-globin gene. It is capable of enhancing the expression of a cis-linked test gene by up to 300-fold. This enhancer element is apparently developmental-stage-independent, as it is functional at the embryonic and the adult developmental stages in erythroid cells that are expressing the respective beta-like globin genes. The enhancer and globin promoter sequences work in synergy and are capable of conferring on a cis-linked gene the high transcriptional efficiency (enhancer function), erythroid specificity (enhancer and promoter functions), and developmental-stage specificity (promoter function) that are characteristic of the in vivo transcription of the beta-like globin genes in erythroid cells.

MeSH Terms
Age Factors Animals Cricetinae Enhancer Elements, Genetic Erythrocytes/physiology Erythropoiesis Gene Expression Regulation Globins/genetics HeLa Cells Humans Restriction Mapping
Chemicals
Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tuan D Y
Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge 02139.
Solomon W B
London I M
Lee D P
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.8.2554
Published
1989-04-00
Pages
2554-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286955
Subset
IM
Grants
NIDDK NIH HHS · DK 16272 · United States
NHLBI NIH HHS · HL 39948 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.8.2554 PMC Full Text View journal details
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