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PMID: 3879975 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The "beta-like-globin" gene domain in human erythroid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 19 ·1985-10-00 ·Pages 6384-8

Tuan D, Solomon W, Li Q, London IM

Abstract

We have mapped the distribution of the major and minor DNase I-hypersensitive sites in the human "beta-like-globin" gene domain. The minor DNase I-hypersensitive sites map close to the 5' end of each of the beta-like-globin genes. Their presence is specifically associated with the transcription of the immediate downstream beta-like-globin genes. The major DNase I-hypersensitive sites map in what appear to be the 5' and 3' boundary areas of the human beta-like-globin gene domain, a region estimated to span at least 90 kilobases of DNA. These major sites are present in various erythroid cells, which express predominantly either the embryonic, the fetal, or the adult beta-like-globin genes, and seem to be involved in defining the active beta-like-globin genes domain in cells of erythroid lineage. The four major DNase I-hypersensitive sites in the 5' boundary area, when correlated with sequencing data, are shown to be located in DNA regions containing enhancer core-like sequences and alternating purine and pyrimidine bases.

MeSH Terms
Base Sequence Bone Marrow/pathology Cell Line DNA/metabolism Deoxyribonuclease I/metabolism Gene Expression Regulation Globins/genetics Humans Leukemia, Erythroblastic, Acute/genetics Leukemia, Myeloid/genetics Leukemia, Myeloid, Acute/genetics Sequence Homology, Nucleic Acid
Chemicals
Globins DNA Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tuan D
Solomon W
Li Q
London I M
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.19.6384
Published
1985-10-00
Pages
6384-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390720
Subset
IM
Grants
NIADDK NIH HHS · AM16272 · United States
NIADDK NIH HHS · AM20120 · United States
NIGMS NIH HHS · GM20069 · United States
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GENBANK
J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067
External Links
PubMed source DOI: 10.1073/pnas.82.19.6384 PMC Full Text View journal details
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