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PMID: 3480506 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for a locus activation region: the formation of developmentally stable hypersensitive sites in globin-expressing hybrids.

Nucleic acids research ·Vol. 15 ·No. 24 ·1987-12-23 ·Pages 10159-77

Forrester WC, Takegawa S, Papayannopoulou T, Stamatoyannopoulos G, Groudine M

Abstract

We have analyzed the chromatin structure of the human beta-globin locus in somatic cell hybrids resulting from the fusion of human non-erythroid cells and mouse erythroleukemia (MEL) cells. In these hybrids, the human adult beta-globin gene, but neither the embryonic nor fetal globin genes, is activated transcriptionally. In addition, the DNase I-resistant beta-like globin locus characteristic of the parental non-erythroid human cells (1,2) is reorganized over an approximately 80 kb region, including the formation of the developmentally stable hypersensitive sites 50 kb 5' and 20 kg 3' of the activated adult beta-globin gene (2,3). These results are consistent with the hypothesis that events occurring at the 5' and/or 3' developmentally stable hypersensitive sites are important, if not necessary, for the activation of the beta-globin locus.

MeSH Terms
Animals Chromatin/ultrastructure Deoxyribonuclease I Fibroblasts/physiology Gene Expression Regulation Globins/genetics Humans Hybrid Cells Leukemia, Erythroblastic, Acute/genetics Mice Multigene Family Regulatory Sequences, Nucleic Acid
Chemicals
Chromatin Globins Deoxyribonuclease I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Forrester W C
Division of Basic Sciences, Genetics, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Takegawa S
Papayannopoulou T
Stamatoyannopoulos G
Groudine M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.24.10159
Published
1987-12-23
Pages
10159-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339937
Subset
IM
Grants
NCI NIH HHS · 2-T32-CA09437-03 · United States
NIADDK NIH HHS · AM31232 · United States
NIDDK NIH HHS · DK30852 · United States
External Links
PubMed source DOI: 10.1093/nar/15.24.10159 PMC Full Text View journal details
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