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PMID: 2347301 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development.

The EMBO journal ·Vol. 9 ·No. 6 ·1990-06-00 ·Pages 1677-84

Benfey PN, Ren L, Chua NH

Abstract

The cauliflower mosaic virus (CaMV) 35S enhancer is able to confer strong constitutive expression in plants. We have previously defined two domains within this enhancer that can confer different tissue-specific expression patterns throughout development. We show here that the upstream domain (B) has a modular organization. It contains at least five subdomains that are able to confer distinct expression patterns when fused to the downstream domain (A). When fused to a minimal promoter only three of the five subdomains give any expression in the early stages of plant development. Comparison of the expression patterns conferred by the subdomains alone, in combination with the downstream domain or in combination with other subdomains provides evidence for synergistic interactions among cis-elements within the 35S enhancer.

MeSH Terms
Base Sequence Enhancer Elements, Genetic Gene Expression Regulation, Viral Genetic Variation Molecular Sequence Data Mosaic Viruses/genetics Nucleic Acid Conformation Plant Cells Plant Development Plants/genetics,microbiology Seeds/cytology,genetics,growth & development,microbiology Transformation, Genetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Benfey P N
Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021.
Ren L
Chua N H
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19 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-06-00
Pages
1677-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551870
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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