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PMID: 1653221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lambda Gam protein inhibits the helicase and chi-stimulated recombination activities of Escherichia coli RecBCD enzyme.

Journal of bacteriology ·Vol. 173 ·No. 18 ·1991-09-00 ·Pages 5808-21

Murphy KC

Abstract

The lambda Gam protein was isolated from cells containing a Gam-producing plasmid. The purified Gam protein was found to bind to RecBCD without displacing any of its subunits. Gam was shown to inhibit all known enzymatic activities of RecBCD: ATP-dependent single- and double-stranded DNA exonucleases, ATP-independent single-stranded endonuclease, and the ATP-dependent helicase. When produced in vivo, Gam inhibited chi-activated recombination in lambda red gam crosses but had little effect on the host's ability to act as a recipient in conjugational recombination. These experiments suggest that RecBCD possesses an additional "unknown" activity that is resistant to or induced by Gam. Additionally, the expression of Gam in recD mutants sensitizes the host to UV irradiation, indicating that Gam alters one or more of the in vivo activities of RecBC(D-).

Related Genes
gamL gamS recB recC recD sbcC
MeSH Terms
Amino Acid Sequence Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Conjugation, Genetic DNA Helicases/antagonists & inhibitors DNA Repair DNA Replication DNA, Bacterial/radiation effects DNA, Viral/genetics DNA-Binding Proteins/pharmacology Escherichia coli/genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/antagonists & inhibitors,metabolism Molecular Sequence Data Recombination, Genetic/drug effects Ultraviolet Rays Viral Proteins/pharmacology
Chemicals
DNA, Bacterial DNA, Viral DNA-Binding Proteins Escherichia coli Proteins Viral Proteins gam protein, Coliphage Exodeoxyribonucleases Exodeoxyribonuclease V exodeoxyribonuclease V, E coli DNA Helicases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Murphy K C
Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester 01605.
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.18.5808-5821.1991
Published
1991-09-00
Pages
5808-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208314
Subset
IM
Grants
NIAID NIH HHS · AI18234 · United States
External Links
PubMed source DOI: 10.1128/jb.173.18.5808-5821.1991 PMC Full Text View journal details
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