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PMID: 6317651 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12.

Journal of bacteriology ·Vol. 157 ·No. 1 ·1984-01-00 ·Pages 21-7

Dykstra CC, Prasher D, Kushner SR

Abstract

A 19-kilobase BamHI fragment encoding the recB (exonuclease V), recC (exonuclease V), ptr (protease III), thyA, and argA genes of Escherichia coli K-12 was cloned into a multicopy plasmid (pCDK3). In E. coli maxicells, the plasmid specified the synthesis of seven polypeptides of 140,000 (recC), 128,000 (recB), 110,000 (ptr), 53,000 (argA), 50,000, 33,000 (thyA), and 22,000 Mr, as well as beta-lactamase and chloramphenicol acetyltransferase. From analysis of subclones and Tn1000 insertions, it appears that the 110,000- and 50,000-Mr proteins originated from the ptr DNA coding sequence which is located between the recB and recC genes. Although recC, ptr, and recB were physically closely linked and transcribed in the same direction, they do not appear to constitute an operon. Cells carrying pCDK3 contained a 30- to 50-fold increase in exonuclease V activity, without affecting cell viability.

MeSH Terms
Cloning, Molecular/methods DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/analysis,genetics Exonucleases/genetics Genes, Bacterial Mutation Peptide Biosynthesis Peptides/genetics Plasmids Transcription, Genetic
Chemicals
DNA Transposable Elements DNA, Bacterial Peptides Exonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dykstra C C
Prasher D
Kushner S R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.157.1.21-27.1984
Published
1984-01-00
Pages
21-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215123
Subset
IM
Grants
NIGMS NIH HHS · GM27997 · United States
NIGMS NIH HHS · GM28760 · United States
External Links
PubMed source DOI: 10.1128/jb.157.1.21-27.1984 PMC Full Text View journal details
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