Home LiteratureArticle Details
PMID: 2177520 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Linear multimer formation of plasmid DNA in Escherichia coli hopE (recD) mutants.

Molecular & general genetics : MGG ·Vol. 224 ·No. 1 ·1990-10-00 ·Pages 1-9

Niki H, Ogura T, Hiraga S

Abstract

The hopE mutants of Escherichia coli, which cannot stably maintain a mini-F plasmid during cell division, have mutations in the recD gene coding for subunit D of the RecBCD enzyme (exonuclease V). A large amount of linear multimer DNA of mini-F and pBR322 plasmid accumulates in these hopE mutants. The linear multimers of plasmid DNA in the hopE (recD) mutants accumulate in sbc+ genetic backgrounds and this depends on the recA+ gene function. Linear plasmid multimers also accumulated in a recBC xthA triple mutant, but not an isogenic xth A mutant or an isogenic recBC mutant. The recBC xth A mutant is defective in the conjugative type of recombination. Linear plasmid multimers were not detected in the recBC strain. We propose models to account for linear multimer formation of plasmids in various mutants.

Related Genes
recA recBC recD
MeSH Terms
Chromosome Mapping DNA Helicases/genetics DNA Replication DNA, Bacterial/genetics Escherichia coli/enzymology,genetics Escherichia coli Proteins Exodeoxyribonuclease V Exodeoxyribonucleases/genetics F Factor Genes, Bacterial Models, Genetic Mutation Rec A Recombinases/genetics
Chemicals
DNA, Bacterial Escherichia coli Proteins Rec A Recombinases Exodeoxyribonucleases Exodeoxyribonuclease V exodeoxyribonuclease V, E coli DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Niki H
Department of Molecular Genetics, Kumamoto University Medical School, Japan.
Ogura T
Hiraga S
References (44)
44 references, click to expand
  1. Enzymatic DNA degradation in E. coli: its relationship to synthetic processes at the chromosome level.
    Cold Spring Harb Symp Quant Biol. 1968;33:259-69 PMID: 4891968
  2. Synthesis of linear multimers of OriC and pBR322 derivatives in Escherichia coli K-12: role of recombination and replication functions.
    J Bacteriol. 1987 Jul;169(7):3131-7 PMID: 3298213
  3. Site-specific and illegitimate recombination in the oriV1 region of the F factor. DNA sequences involved in recombination and resolution.
    J Mol Biol. 1986 May 5;189(1):85-102 PMID: 3023624
  4. A new class of Escherichia coli recBC mutants: implications for the role of RecBC enzyme in homologous recombination.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7850-4 PMID: 6393130
  5. Isolation of exonuclease VIII: the enzyme associated with sbcA indirect suppressor.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3593-7 PMID: 4610579
  6. Recombination-deficient mutations and thymineless death in Escherichia coli K12: reciprocal effects of recBC and recF and indifference of recA mutations.
    Can J Microbiol. 1982 Apr;28(4):425-30 PMID: 7046891
  7. Complete nucleotide sequence of recD, the structural gene for the alpha subunit of Exonuclease V of Escherichia coli.
    Nucleic Acids Res. 1986 Nov 11;14(21):8583-94 PMID: 3537961
  8. Control of cell division by sex factor F in Escherichia coli. II. Identification of genes for inhibitor protein and trigger protein on the 42.84-43.6 F segment.
    J Mol Biol. 1984 Apr 25;174(4):627-46 PMID: 6233425
  9. Homologous recombination in procaryotes.
    Microbiol Rev. 1988 Mar;52(1):1-28 PMID: 3280962
  10. Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12.
    J Bacteriol. 1985 Nov;164(2):836-44 PMID: 3932331
  11. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  12. Recombination deficient mutants of E. coli and other bacteria.
    Annu Rev Genet. 1973;7:67-86 PMID: 4205905
  13. Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12.
    J Bacteriol. 1976 Jun;126(3):1082-8 PMID: 780339
  14. Fine structure of the recB and recC gene region of Escherichia coli.
    Biochem Biophys Res Commun. 1982 Nov 30;109(2):414-22 PMID: 6295393
  15. Chi-stimulated patches are heteroduplex, with recombinant information on the phage lambda r chain.
    Cell. 1987 Mar 13;48(5):855-65 PMID: 2949853
  16. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
    J Mol Biol. 1975 May 25;94(3):441-8 PMID: 1100841
  17. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  18. The isolation and characterization from Escherichia coli of an adenosine triphosphate-dependent deoxyribonuclease directed by rec B, C genes.
    J Biol Chem. 1971 Nov;246(21):6543-55 PMID: 4332130
  19. Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli.
    J Bacteriol. 1985 Sep;163(3):1060-6 PMID: 2993230
  20. Chromosomal genes essential for stable maintenance of the mini-F plasmid in Escherichia coli.
    J Bacteriol. 1988 Nov;170(11):5272-8 PMID: 3053654
  21. Mutations in the recD gene of Escherichia coli that raise the copy number of certain plasmids.
    J Bacteriol. 1987 Oct;169(10):4841-4 PMID: 3308857
  22. Characterization of the deoxyribonuclease determined by lambda reverse as exonuclease VIII of Escherichia coli.
    J Mol Biol. 1977 Jun 15;113(1):27-41 PMID: 328898
  23. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination.
    Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292-9 PMID: 4916924
  24. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  25. Determination of plasmid copy number by fluorescence densitometry.
    Plasmid. 1983 Mar;9(2):182-90 PMID: 6344110
  26. Genetic analysis of regulation of the RecF pathway of recombination in Escherichia coli K-12.
    J Bacteriol. 1983 Mar;153(3):1471-8 PMID: 6337999
  27. Identification and characterization of recD, a gene affecting plasmid maintenance and recombination in Escherichia coli.
    J Bacteriol. 1986 Aug;167(2):594-603 PMID: 3015881
  28. The genetic dependence of recombination in recD mutants of Escherichia coli.
    Genetics. 1988 Sep;120(1):37-45 PMID: 3065139
  29. Exonucleases I, III, and V are required for stability of ColE1-related plasmids in Escherichia coli.
    J Bacteriol. 1984 Feb;157(2):661-4 PMID: 6363393
  30. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  31. Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):688-92 PMID: 1091930
  32. 3' homologous free ends are required for stable joint molecule formation by the RecA and single-stranded binding proteins of Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):690-4 PMID: 3543934
  33. Modulation of the action of the recBC enzyme of Escherichia coli K-12 by Ca2+.
    J Biol Chem. 1979 Sep 10;254(17):8646-52 PMID: 157358
  34. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):955-61 PMID: 4909471
  35. Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.
    J Biol Chem. 1972 Jan 10;247(1):241-8 PMID: 4336040
  36. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  37. recD: the gene for an essential third subunit of exonuclease V.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5558-62 PMID: 3526335
  38. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  39. Effect of recF, recJ, recN, recO and ruv mutations on ultraviolet survival and genetic recombination in a recD strain of Escherichia coli K12.
    Mol Gen Genet. 1988 May;212(2):317-24 PMID: 2841571
  40. The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation.
    Mol Gen Genet. 1986 Nov;205(2):298-304 PMID: 3027506
  41. Exonuclease VIII of Escherichia coli. II. Mechanism of action.
    J Biol Chem. 1983 Sep 10;258(17):10418-24 PMID: 6309804
  42. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  43. Mini-F plasmid genes that couple host cell division to plasmid proliferation.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4784-8 PMID: 6308648
  44. Synthesis of linear plasmid multimers in Escherichia coli K-12.
    J Bacteriol. 1986 Jul;167(1):327-35 PMID: 3522548
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00259444
Published
1990-10-00
Pages
1-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00259444
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2