Home LiteratureArticle Details
PMID: 1650910 Published · ppublish English Journal Article

Selective cloning of genes encoding RNase H from Salmonella typhimurium, Saccharomyces cerevisiae and Escherichia coli rnh mutant.

Molecular & general genetics : MGG ·Vol. 227 ·No. 3 ·1991-07-00 ·Pages 438-45

Itaya M, McKelvin D, Chatterjie SK, Crouch RJ

Abstract

We have cloned genes encoding RNase H from Escherichia coli rnh mutants, Salmonella typhimurium and Saccharomyces cerevisiae. Selection was accomplished by suppression of the temperature-sensitive growth phenotype of Escherichia coli strains containing the rnh-339::cat and either recB270 (Ts) or recC271 (Ts) mutations. RNases H from E. coli and S. typhimurium contained 155 amino acid residues and differed at only 11 positions. The S. cerevisiae and E. coli RNases H were about 30% homologous. A comparison of the amino acid sequences of several RNases H from cellular and retroviral sources revealed some strongly conserved regions as well as variable regions; the carboxyl-terminus was particularly variable. The overlapping, divergent promoter gene organization found in E. coli was observed to be present in S. typhimurium.

Related Genes
rnh
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Endoribonucleases/genetics Escherichia coli/genetics Genes, Bacterial Genes, Fungal Molecular Sequence Data Mutation Nucleic Acid Hybridization Phenotype Plasmids Promoter Regions, Genetic Ribonuclease H Saccharomyces cerevisiae/genetics Salmonella typhimurium/genetics Sequence Homology, Nucleic Acid
Chemicals
Endoribonucleases Ribonuclease H
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Itaya M
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
McKelvin D
Chatterjie S K
Crouch R J
References (40)
40 references, click to expand
  1. Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia virus reverse transcriptase.
    J Virol. 1989 Mar;63(3):1460-4 PMID: 2464706
  2. Domain structure of the Moloney murine leukemia virus reverse transcriptase: mutational analysis and separate expression of the DNA polymerase and RNase H activities.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1777-81 PMID: 2450347
  3. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  4. Role of DNA-RNA hybrids in eukaryotes. Ribonuclease H in yeast.
    Eur J Biochem. 1973 Jun;35(2):270-81 PMID: 4123953
  5. Three-dimensional structure of ribonuclease H from E. coli.
    Nature. 1990 Sep 20;347(6290):306-9 PMID: 1698262
  6. Transcriptional organization of the convergent overlapping dnaQ-rnh genes of Escherichia coli.
    J Biol Chem. 1985 Jun 10;260(11):7122-5 PMID: 2987244
  7. The structure of transposable yeast mating type loci.
    Cell. 1980 Mar;19(3):753-64 PMID: 6244896
  8. Structure of ribonuclease H phased at 2 A resolution by MAD analysis of the selenomethionyl protein.
    Science. 1990 Sep 21;249(4975):1398-405 PMID: 2169648
  9. Isolation of cloned Moloney murine leukemia virus reverse transcriptase lacking ribonuclease H activity.
    Nucleic Acids Res. 1988 Jan 11;16(1):265-77 PMID: 2448747
  10. A combination of RNase H (rnh) and recBCD or sbcB mutations in Escherichia coli K12 adversely affects growth.
    Mol Gen Genet. 1991 Jul;227(3):424-32 PMID: 1650908
  11. Nucleotide sequence of Rous sarcoma virus.
    Cell. 1983 Mar;32(3):853-69 PMID: 6299578
  12. Absence of RNase H allows replication of pBR322 in Escherichia coli mutants lacking DNA polymerase I.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7845-9 PMID: 6096862
  13. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes.
    Genetics. 1973 May;74(1):33-54 PMID: 17248609
  14. Nucleotide sequence of AKV murine leukemia virus.
    J Virol. 1984 Feb;49(2):471-8 PMID: 6319746
  15. Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes.
    Mol Cell Biol. 1989 Jul;9(7):2989-99 PMID: 2674676
  16. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  17. Low levels of RNase H activity in Escherichia coli FB2 rnh result from a single-base change in the structural gene of RNase H.
    J Bacteriol. 1983 May;154(2):1021-6 PMID: 6302075
  18. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  19. Identification of the amino acid residues involved in an active site of Escherichia coli ribonuclease H by site-directed mutagenesis.
    J Biol Chem. 1990 Mar 15;265(8):4615-21 PMID: 1689729
  20. DNA sequence and coding properties of mutD(dnaQ) a dominant Escherichia coli mutator gene.
    J Mol Biol. 1986 Jul 5;190(1):113-7 PMID: 3023634
  21. In vivo studies of temperature-sensitive recB and recC mutants.
    J Bacteriol. 1974 Dec;120(3):1213-8 PMID: 4612007
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Functional organization of the murine leukemia virus reverse transcriptase: characterization of a bacterially expressed AKR DNA polymerase deficient in RNase H activity.
    J Virol. 1988 Nov;62(11):4376-80 PMID: 2459414
  24. In addition to RNase H(70) two other proteins of Saccharomyces cerevisiae exhibit ribonuclease H activity.
    J Biol Chem. 1988 Oct 15;263(29):14970-7 PMID: 3049596
  25. Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7648-52 PMID: 2429313
  26. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  27. Structural genes of ATP-dependent deoxyribonuclease of Escherichia coli.
    Nat New Biol. 1972 Sep 6;239(88):14-6 PMID: 4562227
  28. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  29. Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli.
    Mol Gen Genet. 1984;193(2):231-7 PMID: 6319961
  30. The rnh gene is essential for growth of Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3447-51 PMID: 6233609
  31. Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast.
    Nucleic Acids Res. 1985 Sep 25;13(18):6679-93 PMID: 2997719
  32. DNA sequence of the gene coding for Escherichia coli ribonuclease H.
    J Biol Chem. 1983 Jan 25;258(2):1276-81 PMID: 6296074
  33. Identification and characterization of HIV-specific RNase H by monoclonal antibody.
    EMBO J. 1988 Jan;7(1):239-43 PMID: 2452083
  34. Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7137-41 PMID: 6316347
  35. Reduced transcription of the rnh gene in Escherichia coli mutants expressing the SOS regulon constitutively.
    Mol Gen Genet. 1987 Jan;206(1):95-100 PMID: 3033443
  36. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  37. Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H.
    Mol Gen Genet. 1991 Jul;227(3):433-7 PMID: 1650909
  38. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  39. Differential thermolability of exonuclease and endonuclease activities of the recBC nuclease isolated from thermosensitive recB and recC mutants.
    J Bacteriol. 1974 Dec;120(3):1219-22 PMID: 4612008
  40. Ty, an endogenous retrovirus of yeast?
    Yeast. 1986 Sep;2(3):145-52 PMID: 3333303
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00273935
Published
1991-07-00
Pages
438-45
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
S47099, S47111, S47117, X63981, X63982, X63983, X63984, X63985, X63986, X63987
External Links
PubMed source DOI: 10.1007/BF00273935
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2