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PMID: 2429313 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 20 ·1986-10-00 ·Pages 7648-52

Johnson MS, McClure MA, Feng DF, Gray J, Doolittle RF

Abstract

A computer analysis of the amino acid sequences from the putative gene products of retroviral pol genes has revealed a 150-residue segment that is homologous with the ribonuclease H of Escherichia coli. The segment occurs at the carboxyl terminus of the region assigned to the 90-kDa reverse transcriptase polypeptide. In contrast, a section nearer the amino terminus of this sequence can be aligned with nonretroviral polymerases. The order of activities in the pol gene is thus: polymerase-ribonuclease-endonuclease. On another note, all retroviral endonuclease sequences contain a consensus zinc-binding "finger." This should not be confused with the well-known zinc requirement of reverse transcriptases.

MeSH Terms
Amino Acid Sequence Computers DNA Helicases/analysis Endoribonucleases/analysis Genes, Viral Integrases RNA-Directed DNA Polymerase/analysis Retroviridae/enzymology,genetics Ribonuclease H Viral Proteins/analysis
Chemicals
Viral Proteins Integrases RNA-Directed DNA Polymerase Endoribonucleases Ribonuclease H DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Johnson M S
McClure M A
Feng D F
Gray J
Doolittle R F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.20.7648
Published
1986-10-00
Pages
7648-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386778
Subset
IM
Grants
NIGMS NIH HHS · GM-34434 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.20.7648 PMC Full Text View journal details
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