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PMID: 16453485 Published · ppublish English Journal Article

Molecular cloning of the avian acute transforming retrovirus MH2 reveals a novel cell-derived sequence (v-mil) in addition to the myc oncogene.

The EMBO journal ·Vol. 2 ·No. 12 ·1983-00-00 ·Pages 2189-94

Coll J, Righi M, Taisne C, Dissous C, Gegonne A, Stehelin D

Abstract

Mill-Hill-2 virus (MH2) proviral DNA was cloned from a transformed non-producer cell culture (MH2QB2) through insertion of randomly cut high mol. wt. cellular DNA in the lambdoid vector L47.1. Restriction analysis of a suitable recombinant phage by Southern DNA blotting and hybridization with different probes allowed us to characterize the genetic organization of the provirus and to identify a novel MH2-specific sequence of at least 1.1kbp. Such a sequence, for which we propose the name v-mil, from MilI-Hill-2 virus, is not homologous to v-myc, the previously described oncogene of MH2, nor to avian leukaemia virus-related sequences. Evidence is presented here that v-mil has a cellular counterpart (c-mil) phylogenetically conserved in birds and mammals, including man, and expressed as a single RNA species at least in some tissues. MH2 virus might thus be regarded, like avian erythroblastosis virus or E26, as another example of retroviruses having recombined with more than one cellular gene.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Coll J
INSERM U 186, Oncologie Moleculaire, Institut Pasteur, 15 Rue C. Guerin, 59019 Lille Cedex, France.
Righi M
Taisne C
Dissous C
Gegonne A
Stehelin D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
2189-94
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555433
External Links
PubMed source PMC Full Text View journal details
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