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PMID: 6296427 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA and protein encoded by MH2 virus: evidence for subgenomic expression of v-myc.

Journal of virology ·Vol. 45 ·No. 1 ·1983-01-00 ·Pages 133-9

Pachl C, Biegalke B, Linial M

Abstract

MH2 and MC29 are highly related myc-containing avian retroviruses. We found that MH2, unlike MC29, synthesizes a 2.6-kilobase subgenomic mRNA containing myc sequences as well as sequences from the 5' end of the genome. A 57-kilodalton protein containing myc, but not gag, sequences (p57myc) was detected by hybrid selection and in vitro translation of RNA from MH2-transformed cells. Gradient separation of MH2 intracellular RNAs indicated that p57myc is encoded by the subgenomic RNA. A highly oncogenic MH2 virus variant (MH2YS3) (M. Linial, Virology 119:382-391, 1982) was shown to encode only p57myc and not P100, the previously described MH2-encoded polyprotein (Hu et al., Virology, 89:162-178, 1978). Cells transformed by subclones of this virus synthesized predominantly the 2.6-kilobase RNA rather than genomic 5.4-kilobase RNA. These results suggest that only p57myc is required for maintenance of the transformed state after MH2 infection.

MeSH Terms
Animals Avian Leukosis Virus/genetics,physiology Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral Genes, Viral Oncogenes Protein Biosynthesis Quail RNA, Messenger/genetics RNA, Viral/genetics Viral Proteins/genetics
Chemicals
RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pachl C
Biegalke B
Linial M
References (28)
28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.45.1.133-139.1983
Published
1983-01-00
Pages
133-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256394
Subset
IM
Grants
NCI NIH HHS · CA-18282 · United States
NCI NIH HHS · CA-28151 · United States
External Links
PubMed source DOI: 10.1128/JVI.45.1.133-139.1983 PMC Full Text View journal details
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